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anti-PKCdelta antibody :: PKCdelta Antibody

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Catalog # MBS856672
Unit / Price
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  0.05 mg  /  $180 +1 FREE 8GB USB
  0.1 mg  /  $215 +1 FREE 8GB USB
Product Name

PKCdelta, Antibody

Popular Item
Full Product Name

PKCdelta (Ab-645) Antibody

Product Synonym Names
PRKCD; Protein kinase C delta type
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
176977
3D Structure
ModBase 3D Structure for Q05655
Species Reactivity
Human, Mouse, Rat
Specificity
PKCdelta (Ab-645) antibody detects endogenous levels of total PKCdelta protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human PKCdelta around the phosphorylation site of serine 645 (R-L-SP-Y-S).
Preparation and Storage
Store at-20 degree C for 1 year
Other Notes
Small volumes of anti-PKCdelta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PKCdelta antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PKCdelta antibody
WB: 1:500~1:1000
IHC: 1:50~1:100

Immunohistochemistry (IHC) of anti-PKCdelta antibody
Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCdelta (Ab-645) antibody.
anti-PKCdelta antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-PKCdelta antibody
Western blot analysis of extracts from MCF7 cells using PKCdelta (Ab-645) antibody and PKCdelta (phospho-Ser645) antibody.
anti-PKCdelta antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for PKCdelta. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
80,953 Da
NCBI Official Full Name
protein kinase C delta type
NCBI Official Synonym Full Names
protein kinase C delta
NCBI Official Symbol
PRKCD  [Similar Products]
NCBI Official Synonym Symbols
MAY1; PKCD; ALPS3; CVID9; nPKC-delta
  [Similar Products]
NCBI Protein Information
protein kinase C delta type
UniProt Protein Name
Protein kinase C delta type
UniProt Synonym Protein Names
Tyrosine-protein kinase PRKCD (EC:2.7.10.2); nPKC-delta
Protein Family
UniProt Gene Name
PRKCD  [Similar Products]
UniProt Synonym Gene Names
NCBI Summary for PKCdelta
The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome. [provided by RefSeq, Aug 2017]
UniProt Comments for PKCdelta
Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression as well as survival of several cancers, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. Negatively regulates B cell proliferation and also has an important function in self-antigen induced B cell tolerance induction. Upon DNA damage, activates the promoter of the death-promoting transcription factor BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription and apoptosis. In response to oxidative stress, interact with and activate CHUK/IKKA in the nucleus, causing the phosphorylation of p53/TP53. In the case of ER stress or DNA damage-induced apoptosis, can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol can trigger apoptosis by activating MAPK11 or MAPK14, inhibiting AKT1 and decreasing the level of X-linked inhibitor of apoptosis protein (XIAP), whereas in nucleus induces apoptosis via the activation of MAPK8 or MAPK9. Upon ionizing radiation treatment, is required for the activation of the apoptosis regulators BAX and BAK, which trigger the mitochondrial cell death pathway. Can phosphorylate MCL1 and target it for degradation which is sufficient to trigger for BAX activation and apoptosis. Is required for the control of cell cycle progression both at G1/S and G2/M phases. Mediates phorbol 12-myristate 13-acetate (PMA)-induced inhibition of cell cycle progression at G1/S phase by up-regulating the CDK inhibitor CDKN1A/p21 and inhibiting the cyclin CCNA2 promoter activity. In response to UV irradiation can phosphorylate CDK1, which is important for the G2/M DNA damage checkpoint activation. Can protect glioma cells from the apoptosis induced by TNFSF10/TRAIL, probably by inducing increased phosphorylation and subsequent activation of AKT1. Is highly expressed in a number of cancer cells and promotes cell survival and resistance against chemotherapeutic drugs by inducing cyclin D1 (CCND1) and hyperphosphorylation of RB1, and via several pro-survival pathways, including NF-kappa-B, AKT1 and MAPK1/3 (ERK1/2). Can also act as tumor suppressor upon mitogenic stimulation with PMA or TPA. In N-formyl-methionyl-leucyl-phenylalanine (fMLP)-treated cells, is required for NCF1 (p47-phox) phosphorylation and activation of NADPH oxidase activity, and regulates TNF-elicited superoxide anion production in neutrophils, by direct phosphorylation and activation of NCF1 or indirectly through MAPK1/3 (ERK1/2) signaling pathways. May also play a role in the regulation of NADPH oxidase activity in eosinophil after stimulation with IL5, leukotriene B4 or PMA. In collagen-induced platelet aggregation, acts a negative regulator of filopodia formation and actin polymerization by interacting with and negatively regulating VASP phosphorylation. Downstream of PAR1, PAR4 and CD36/GP4 receptors, regulates differentially platelet dense granule secretion; acts as a positive regulator in PAR-mediated granule secretion, whereas it negatively regulates CD36/GP4-mediated granule release. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. The catalytic subunit phosphorylates 14-3-3 proteins (YWHAB, YWHAZ and YWHAH) in a sphingosine-dependent fashion (). Phosphorylates ELAVL1 in response to angiotensin-2 treatment (PubMed:18285462).
Product References and Citations for anti-PKCdelta antibody
Kei Sakamoto, et, al. (2003) Am J Physiol Endocrinol Metab, ; 285: E1081-E1088. Ling Zhang, et, al. (2004) J. Biol. Chem; 279: 28315-28319. Kristof Van Kolen et, al. (2006) FEBS J; 273: 1843-1854. Martin Villalba, et, al. (2002) J. Cell Biol; 157: 253.

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