• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PKLR elisa kit :: Dog pyruvate kinase, liver and RBC ELISA Kit

Scan QR to view Datasheet
Catalog # MBS920482
Unit / Price DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Typical Testing Data/Standard Curve (for reference only)
Product Name

pyruvate kinase, liver and RBC (PKLR), ELISA Kit

Popular Item
Also Known As

Dog Pyruvate kinase isozymes R/L, PKLR ELISA Kit

Product Synonym Names
Dog Pyruvate kinase isozymes R/L (PKLR) ELISA kit; PK1; PKL; PKR; PKRL; RPK; R-type/L-type pyruvate kinase; pyruvate kinase 1; pyruvate kinase isozyme R/L; pyruvate kinase type L; pyruvate kinase; liver and blood cell; red cell/liver pyruvate kinase; pyruvate kinase; liver and RBC
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS920482 Testing Data
Sequence Length
543
3D Structure
ModBase 3D Structure for Q29536
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of dog PKLR. No significant cross-reactivity or interference between dog PKLR and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
7.81 mU/ml-500 mU/ml.
Sensitivity
The minimum detectable dose of dog PKLR is typically less than 1.95 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PKLR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPKLRpurchase
MBS920482 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the pyruvate kinase, liver and RBC (PKLR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PKLR. The ELISA analytical biochemical technique of the MBS920482 kit is based on PKLR antibody-PKLR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PKLR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PKLR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PKLR elisa kit
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PKLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PKLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PKLR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PKLR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of PKLR elisa kit
PKLR elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS920482. Click to request current manual
NCBI/Uniprot data below describe general gene information for PKLR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
62,048 Da
NCBI Official Full Name
pyruvate kinase PKLR isoform 2
NCBI Official Symbol
NCBI Protein Information
pyruvate kinase PKLR; Pyruvate kinase isozyme R; Pyruvate kinase isozymes R/L; pyruvate kinase isozymes L/R
UniProt Protein Name
Pyruvate kinase PKLR
UniProt Synonym Protein Names
Pyruvate kinase isozymes L/R
Protein Family
UniProt Gene Name
UniProt Entry Name
KPYR_CANFA
UniProt Comments for PKLR
Function: Plays a key role in glycolysis

Catalytic activity: ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor: Magnesium

Enzyme regulation: Allosterically activated by fructose 1,6-bisphosphate
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with PKLR elisa kit
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000