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PL-A2 elisa kit :: Rat Phospholipase A2 ELISA Kit

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Catalog # MBS044009
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PL-A2 elisa kit
Product Name

Phospholipase A2 (PL-A2), ELISA Kit

Popular Item
Full Product Name

Rat Phospholipase A2 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 5; NC_005104.3 (161024073..161026650). Location: 5q36
3D Structure
ModBase 3D Structure for P14423
Species Reactivity
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Assay Type
Quantitative Sandwich
Detection Range
3.12 ng/ml - 100 ng/ml.
Sensitivity
1.0 ng/ml.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PL-A2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PL-A2purchase
MBS044009 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phospholipase A2 (PL-A2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PL-A2. The ELISA analytical biochemical technique of the MBS044009 kit is based on PL-A2 antibody-PL-A2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PL-A2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PL-A2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PL-A2 elisa kit
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of PLA2 (hereafter termed "analyte") in undiluted original Rat body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS044009. Click to request current manual
NCBI/Uniprot data below describe general gene information for PL-A2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
16,294 Da
NCBI Official Full Name
phospholipase A2, membrane associated
NCBI Official Synonym Full Names
phospholipase A2, group IIA (platelets, synovial fluid)
NCBI Official Symbol
Pla2g2a  [Similar Products]
NCBI Official Synonym Symbols
sPLA2
  [Similar Products]
NCBI Protein Information
phospholipase A2, membrane associated; GIIC sPLA2; platelet phospholipase A2; group IIA phospholipase A2; eted enzyme type IIA phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A
UniProt Protein Name
Phospholipase A2, membrane associated
UniProt Synonym Protein Names
GIIC sPLA2; Group IIA phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A
Protein Family
UniProt Gene Name
Pla2g2a  [Similar Products]
UniProt Entry Name
PA2GA_RAT
NCBI Summary for PL-A2
hydrolyzes acyl group at the sn-2 position of phospholipids, forming non-esterified fatty acids and lysophospholipids; plays a critical role in inflammation; induces proliferation of smooth muscle cells [RGD, Feb 2006]
UniProt Comments for PL-A2
Function: Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Catalytic activity: Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Cofactor: Binds 1 calcium ion per subunit

By similarity.

Subcellular location: Membrane; Peripheral membrane protein.

Miscellaneous: Group II phospholipase A2 is found in many cells and also extracellularly. The membrane-bound and secreted forms are identical and are encoded by a single gene.

Sequence similarities: Belongs to the phospholipase A2 family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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