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PLA2G4A elisa kit :: Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) ELISA Kit

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Catalog # MBS705411
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Typical Testing Data/Standard Curve (for reference only)
Product Name

phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A), ELISA Kit

Popular Item
Full Product Name

Mouse cytosolic phospholipase A2, cPLA2 ELISA Kit

Product Synonym Names
Mouse cytosolic phospholipase A2; cPLA2 ELISA Kit; MGC126350; PLA2G4; cPLA2-alpha; calcium-dependent phospholipid-binding protein; cytosolic phospholipase A2; group IVA; lysophospholipase; phosphatidylcholine 2-acylhydrolase; phospholipase A2; group IVA (cytosolic; calcium-dependent)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS705411 Testing Data
3D Structure
ModBase 3D Structure for P47713
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse cPLA2. No significant cross-reactivity or interference between mouse cPLA2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
62.5 pg/ml -4000 pg/ml.
Sensitivity
The minimum detectable dose of mouse cPLA2 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PLA2G4A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPLA2G4Apurchase
MBS705411 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLA2G4A. The ELISA analytical biochemical technique of the MBS705411 kit is based on PLA2G4A antibody-PLA2G4A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLA2G4A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLA2G4A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PLA2G4A elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for cPLA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cPLA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cPLA2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cPLA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of PLA2G4A elisa kit
PLA2G4A elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS705411. Click to request current manual
NCBI/Uniprot data below describe general gene information for PLA2G4A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
85,222 Da
NCBI Official Full Name
cytosolic phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2, group IVA (cytosolic, calcium-dependent)
NCBI Official Symbol
Pla2g4a  [Similar Products]
NCBI Official Synonym Symbols
cPLA2; Pla2g4; cPLA2alpha
  [Similar Products]
NCBI Protein Information
cytosolic phospholipase A2; cPLA2-alpha; Type IV PLA2; cytosolic PLA2; phospholipase A2, group 4; phospholipase A2 group IVA; Cytosolic phospholipase A2 (CPLA2)
UniProt Protein Name
Cytosolic phospholipase A2
UniProt Synonym Protein Names
Phospholipase A2 group IVAIncluding the following 2 domains:Phospholipase A2 (EC:3.1.1.4)Alternative name(s):Phosphatidylcholine 2-acylhydrolase
Protein Family
UniProt Gene Name
Pla2g4a  [Similar Products]
UniProt Synonym Gene Names
Cpla2; Pla2g4; cPLA2  [Similar Products]
UniProt Entry Name
PA24A_MOUSE
NCBI Summary for PLA2G4A
The protein encoded by this gene is a member of the phospholipase A2 group IV family. This enzyme hydrolyzes membrane phospholipids, thereby releasing the polyunsaturated fatty acid, arachidonic acid. Arachidonic acid is further metabolized into eicosanoids such as leukotrienes, thromboxanes and prostaglandins, that play important roles in regulating diverse biological processes such as inflammatory responses, membrane and actin dynamics, and tumorigenesis. A rise in intracellular calcium levels results in binding of calcium to the C2 domain of this protein, and triggers the translocation from the cytosol to intracellular membranes, including the Golgi apparatus. Disruption of this gene in mice led to decreased levels of eicosonaoids and platelet-activating factor, decreased allergic symptoms, and impaired reproductive ability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]
UniProt Comments for PLA2G4A
Function: Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

Catalytic activity: Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulation: Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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