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PLA2G5 elisa kit :: Monkey Calcium-dependent phospholipase A2 (PLA2G5) ELISA Kit

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Catalog # MBS7205628
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PLA2G5 elisa kit
Product Name

Calcium-dependent phospholipase A2 (PLA2G5), ELISA Kit

Full Product Name

Monkey Calcium-dependent phospholipase A2 (PLA2G5) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
601192
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PLA2G5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PLA2G5purchase
MBS7205628 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calcium-dependent phospholipase A2 (PLA2G5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLA2G5. The ELISA analytical biochemical technique of the MBS7205628 kit is based on PLA2G5 antibody-PLA2G5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLA2G5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLA2G5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for PLA2G5 elisa kit
NCBI/Uniprot data below describe general gene information for PLA2G5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,674 Da
NCBI Official Full Name
calcium-dependent phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2, group V
NCBI Official Symbol
PLA2G5  [Similar Products]
NCBI Official Synonym Symbols
FRFB; GV-PLA2; PLA2-10; hVPLA(2)
  [Similar Products]
NCBI Protein Information
calcium-dependent phospholipase A2; Ca2+-dependent phospholipase A2; phosphatidylcholine 2-acylhydrolase 5
UniProt Protein Name
Calcium-dependent phospholipase A2
UniProt Synonym Protein Names
Group V phospholipase A2; PLA2-10; Phosphatidylcholine 2-acylhydrolase 5
UniProt Gene Name
PLA2G5  [Similar Products]
UniProt Entry Name
PA2G5_HUMAN
NCBI Summary for PLA2G5
This gene is a member of the secretory phospholipase A2 family. It is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for PLA2G5
PLA2G5: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L- alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L- alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle. Defects in PLA2G5 are the cause of fleck retina, familial benign (FRFB). An autosomal recessive condition associated with a distinctive retinal appearance and no apparent visual or electrophysiologic deficits. Affected individuals are asymptomatic, but fundus examination reveals a striking pattern of diffuse, yellow-white, fleck-like lesions extending to the far periphery of the retina but sparing the foveal region. Belongs to the phospholipase A2 family.

Protein type: Lipid Metabolism - arachidonic acid; Lipid Metabolism - ether lipid; Cell surface; Secreted; Lipid Metabolism - linoleic acid; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.4; Secreted, signal peptide; Lipid Metabolism - glycerophospholipid; Phospholipase

Chromosomal Location of Human Ortholog: 1p36-p34

Cellular Component: Golgi apparatus; cell surface; perinuclear region of cytoplasm; extracellular region; plasma membrane

Molecular Function: heparin binding; calcium-dependent phospholipase A2 activity; calcium ion binding

Biological Process: leukotriene biosynthetic process; platelet activating factor biosynthetic process; response to cAMP; phospholipid metabolic process; response to cytokine stimulus; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; arachidonic acid secretion; lipid catabolic process

Disease: Fleck Retina, Familial Benign
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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