• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PLAGL1 elisa kit :: Human Pleiomorphic Adenoma Gene Like Protein 1 (PLAGL1) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS453178
Unit / Price
  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,425 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,710 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Pleiomorphic Adenoma Gene Like Protein 1 (PLAGL1), ELISA Kit

Popular Item
Also Known As

Human Pleiomorphic Adenoma Gene Like Protein 1 (PLAGL1) ELISA Kit

Product Synonym Names
LOT1; ZAC; ZAC1; Zinc finger protein PLAGL1; Lost on transformation 1; Tumor suppressor ZAC
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
463
OMIM
U72621 mRNA
3D Structure
ModBase 3D Structure for Q9UM63
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of PLAGL1. No significant cross-reactivity or interference between PLAGL1 and analogues was observed.
Samples
Tissue homogenates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.064ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLAGL1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLAGL1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PLAGL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPLAGL1purchase
MBS453178 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pleiomorphic Adenoma Gene Like Protein 1 (PLAGL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLAGL1. The ELISA analytical biochemical technique of the MBS453178 kit is based on PLAGL1 antibody-PLAGL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLAGL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLAGL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PLAGL1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PLAGL1 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLAGL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PLAGL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLAGL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLAGL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PLAGL1 elisa kit
PLAGL1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS453178. Click to request current manual
NCBI/Uniprot data below describe general gene information for PLAGL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,668 Da
NCBI Official Full Name
zinc finger protein PLAGL1 isoform 2
NCBI Official Synonym Full Names
PLAG1 like zinc finger 1
NCBI Official Symbol
PLAGL1  [Similar Products]
NCBI Official Synonym Symbols
ZAC; LOT1; ZAC1
  [Similar Products]
NCBI Protein Information
zinc finger protein PLAGL1
UniProt Protein Name
Zinc finger protein PLAGL1
UniProt Synonym Protein Names
Lost on transformation 1; LOT-1; Pleiomorphic adenoma-like protein 1; Tumor suppressor ZAC
Protein Family
UniProt Gene Name
PLAGL1  [Similar Products]
UniProt Synonym Gene Names
LOT1; ZAC; LOT-1  [Similar Products]
NCBI Summary for PLAGL1
This gene encodes a C2H2 zinc finger protein that functions as a suppressor of cell growth. This gene is often deleted or methylated and silenced in cancer cells. In addition, overexpression of this gene during fetal development is thought to be the causal factor for transient neonatal diabetes mellitus (TNDM). Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding two different protein isoforms. The P1 downstream promoter of this gene is imprinted, with preferential expression from the paternal allele in many tissues. [provided by RefSeq, Nov 2015]
UniProt Comments for PLAGL1
Shows weak transcriptional activatory activity. Transcriptional regulator of the type 1 receptor for pituitary adenylate cyclase-activating polypeptide.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000