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PLAT elisa kit :: tPA total antigen assay ELISA Kit

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Catalog # MBS135510
Unit / Price
  1 Kit  /  $540 +1 FREE 8GB USB
  5 Kits  /  $1,980 +2 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

tPA total antigen assay (PLAT), ELISA Kit

Popular Item
Full Product Name

Human tPA total antigen assay ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 8; NC_000008.10 (42032236..42065194, complement). Location: 8p12
OMIM
173370
3D Structure
ModBase 3D Structure for P00750
Host
Multiple
Specificity
These studies are currently in progress. Please contact us for more information.
Form/Format
Complete Kit
Samples
Human plasma and other biological fluids
Intra-assay Precision
These studies are currently in progress. Please contact us for more information.
Inter-assay Precision
These studies are currently in progress. Please contact us for more information.
Preparation and Storage
Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PLAT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPLATpurchase
MBS135510 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Multiple tPA total antigen assay (PLAT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLAT. The ELISA analytical biochemical technique of the MBS135510 kit is based on PLAT antibody-PLAT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLAT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLAT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PLAT elisa kit
Intended Uses: This human tissue-type plasminogen activator (tPA) total antigen assay is intended for the quantitative determination of total tissue plasminogen activator in human plasma and other biological fluids. For research use only.

Principle of the Assay: Human tPA will bind to the affinity purified capture antibody coated on the microtiter plate. Free, latent and complexed tPA will bind to the plate. After appropriate washing steps, monoclonal anti-human tPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the peroxidase conjugated secondary antibody. Following an additional washing step, TMB is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human tPA. Color development is proportional to the concentration of tPA in the samples.

Background: tPA is a serine protease that catalyzes the activation of plasminogen to plasmin [1]. Clinical studies have indicated that high tPA levels may increase the risk for thrombosis [2], whereas decreased levels may cause neuronal plasticity and degeneration [3].

Typical Testing Data/Standard Curve (for reference only) of PLAT elisa kit
PLAT elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS135510. Click to request current manual
NCBI/Uniprot data below describe general gene information for PLAT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
62,917 Da
NCBI Official Full Name
tissue-type plasminogen activator isoform 1 preproprotein
NCBI Official Synonym Full Names
plasminogen activator, tissue
NCBI Official Symbol
NCBI Official Synonym Symbols
TPA; T-PA
  [Similar Products]
NCBI Protein Information
tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA)
UniProt Protein Name
Tissue-type plasminogen activator
UniProt Gene Name
UniProt Synonym Gene Names
t-PA; t-plasminogen activator; tPA  [Similar Products]
UniProt Entry Name
TPA_HUMAN
NCBI Summary for PLAT
This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Comments for PLAT
PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.68; Secreted, signal peptide; Protease; Secreted; Cytoskeletal; Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: extracellular matrix; extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: negative regulation of proteolysis; synaptic transmission, glutamatergic; response to peptide hormone stimulus; fibrinolysis; response to cAMP; smooth muscle cell migration; response to hypoxia; regulation of synaptic plasticity; protein modification process; platelet-derived growth factor receptor signaling pathway; proteolysis; blood coagulation; transmembrane transport; plasminogen activation

Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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