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PLCB1 elisa kit :: Human 1 phosphatidylinositol 4, 5 bisphosphate phosphodiesterase beta 1 ELISA Kit

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Catalog # MBS7226163
Unit / Price
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PLCB1 elisa kit
Product Name

1 phosphatidylinositol 4, 5 bisphosphate phosphodiesterase beta 1 (PLCB1), ELISA Kit

Popular Item
Full Product Name

Human 1 phosphatidylinositol 4, 5 bisphosphate phosphodiesterase beta 1 ELISA Kit

Product Synonym Names
Human 1 phosphatidylinositol 4, 5 bisphosphate phosphodiesterase b 1 ELISA Kit
Product Gene Name
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 613722
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PLCB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PLCB1purchase
MBS7226163 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1 phosphatidylinositol 4, 5 bisphosphate phosphodiesterase beta 1 (PLCB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLCB1. The ELISA analytical biochemical technique of the MBS7226163 kit is based on PLCB1 antibody-PLCB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLCB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLCB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for PLCB1 elisa kit
NCBI/Uniprot data below describe general gene information for PLCB1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
133,703 Da
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform a
NCBI Official Synonym Full Names
phospholipase C, beta 1 (phosphoinositide-specific)
NCBI Official Symbol
PLCB1  [Similar Products]
NCBI Official Synonym Symbols
PLC-I; EIEE12; PI-PLC; PLC154; PLCB1A; PLCB1B; PLC-154
  [Similar Products]
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1; PLC-beta-1; phospholipase C-I; phosphoinositidase C; inositoltrisphosphohydrolase; triphosphoinositide phosphodiesterase; monophosphatidylinositol phosphodiesterase; 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
UniProt Synonym Protein Names
PLC-154; Phosphoinositide phospholipase C-beta-1; Phospholipase C-I; PLC-I; Phospholipase C-beta-1; PLC-beta-1
UniProt Gene Name
PLCB1  [Similar Products]
UniProt Synonym Gene Names
KIAA0581; PLC-I; PLC-beta-1  [Similar Products]
UniProt Entry Name
PLCB1_HUMAN
NCBI Summary for PLCB1
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for PLCB1
PLCB1: an enzyme that catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. Binds to calmodulin. Uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. Activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variant isoforms have been described.

Protein type: Phospholipase; Carbohydrate Metabolism - inositol phosphate; EC 3.1.4.11

Chromosomal Location of Human Ortholog: 20p12

Cellular Component: nuclear membrane; cytoplasm; nuclear chromatin; nuclear speck; nucleus; cytosol

Molecular Function: calmodulin binding; signal transducer activity; phosphatidylinositol-4,5-bisphosphate binding; enzyme binding; protein homodimerization activity; calcium ion binding; phosphoinositide phospholipase C activity; GTPase activator activity; lamin binding

Biological Process: fat cell differentiation; positive regulation of developmental growth; acetylcholine receptor signaling, muscarinic pathway; inositol phosphate metabolic process; positive regulation of interleukin-12 production; glutamate signaling pathway; metabolic process; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; signal transduction; memory; positive regulation of myoblast differentiation; synaptic transmission; positive regulation of embryonic development; insulin-like growth factor receptor signaling pathway; cerebral cortex development; phosphatidylinositol metabolic process; negative regulation of transcription, DNA-dependent; G2/M transition of mitotic cell cycle; lipid catabolic process; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity

Disease: Epileptic Encephalopathy, Early Infantile, 12
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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