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PLCD4 elisa kit :: Human 1 phosphatidylinositol 4, 5 bisphosphate phosphodiesterase delta 4 ELISA Kit

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Catalog # MBS723031
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
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PLCD4 elisa kit
Product Name

1 phosphatidylinositol 4, 5 bisphosphate phosphodiesterase delta 4 (PLCD4), ELISA Kit

Popular Item
Full Product Name

Human 1 phosphatidylinositol 4, 5 bisphosphate phosphodiesterase delta 4 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
605939
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PLCD4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PLCD4purchase
MBS723031 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1 phosphatidylinositol 4, 5 bisphosphate phosphodiesterase delta 4 (PLCD4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLCD4. The ELISA analytical biochemical technique of the MBS723031 kit is based on PLCD4 antibody-PLCD4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLCD4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLCD4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for PLCD4 elisa kit
NCBI/Uniprot data below describe general gene information for PLCD4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
31,448 Da
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4
NCBI Official Synonym Full Names
phospholipase C, delta 4
NCBI Official Symbol
PLCD4  [Similar Products]
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4; PLC delta4; phosphoinositide phospholipase C-delta-4; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-4
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4
UniProt Synonym Protein Names
Phosphoinositide phospholipase C-delta-4; Phospholipase C-delta-4; PLC-delta-4
UniProt Gene Name
PLCD4  [Similar Products]
UniProt Synonym Gene Names
hPLCD4; PLC-delta-4  [Similar Products]
UniProt Entry Name
PLCD4_HUMAN
NCBI Summary for PLCD4
This gene encodes a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer. [provided by RefSeq, Jan 2011]
UniProt Comments for PLCD4
PLCD4: Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida- induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Phospholipase; EC 3.1.4.11; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nuclear membrane; endoplasmic reticulum; cytoplasm; plasma membrane

Molecular Function: signal transducer activity; calcium ion binding; phosphoinositide phospholipase C activity

Biological Process: acrosome reaction; phosphatidylinositol metabolic process; lipid catabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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