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PLCE1 elisa kit :: Rat 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase epsilon-1 ELISA Kit

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Catalog # MBS2604991
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  48-Strip-Wells  /  $275 +1 FREE 8GB USB
  96-Strip-Wells  /  $435 +1 FREE 8GB USB
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  10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
PLCE1 elisa kit
Product Name

1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase epsilon-1 (PLCE1), ELISA Kit

Full Product Name

Rat 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase epsilon-1 (PLCE1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q8K4S1
Species Reactivity
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PLCE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PLCE1purchase
MBS2604991 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase epsilon-1 (PLCE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLCE1. The ELISA analytical biochemical technique of the MBS2604991 kit is based on PLCE1 antibody-PLCE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLCE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLCE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for PLCE1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
255,047 Da
NCBI Official Full Name
1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase epsilon-1, partial
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
UniProt Synonym Protein Names
Phosphoinositide phospholipase C-epsilon-1; Phospholipase C-epsilon-1; PLC-epsilon-1
UniProt Gene Name
Plce1  [Similar Products]
UniProt Synonym Gene Names
Kiaa1516; Plce; PLC-epsilon-1  [Similar Products]
UniProt Entry Name
PLCE1_MOUSE
UniProt Comments for PLCE1
PLCE1: a widely expressed bifunctional enzyme with phospholipase activity and Ras guanine-exchange factor (RasGEF) activity. Catalyzes the release of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) from 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. Is an effector of heterotrimeric G protein activation. May play a role in cell survival, cell growth, actin organization and T-cell activation. Activated by the heterotrimeric G-protein subunits GNA12, GNA13 and GNB1-GNG2. Activated by HRAS, RAP1A, RHOA, RHOB, RHOC, RRAS and RRAS2. Activated by the G(s)-coupled GPCRs ADRB2, PTGER1 and CHRM3 through cyclic-AMP formation and RAP2B activation. Inhibited by G(i)-coupled GPCRs. Interacts with RHOA. Interacts with IQGAP1, HRAS, RAP1A, RAP2A, RAP2B and RRAS. Recruited to plasma membrane by activated HRAS and RAP2. Recruited to perinuclear membrane by activated RAP1A. Requires calcium for activiation. The Ras-associating domain 1 is degenerate and may not bind HRAS. The Ras-associating domain 2 mediates interaction with GTP-bound HRAS, RAP1A, RAP2A and RAP2B and recruitment of HRAS to the cell membrane. The Ras-GEF domain has a GEF activity towards HRAS and RAP1A. Mediates activation of the mitogen-activated protein kinase pathway. Defects in PLCE1 are the cause of nephrotic syndrome type 3 (NPHS3). Nephrotic syndrome, a malfunction of the kidney glomerular filter, leads to proteinuria, hypoalbuminemia, edema. Overexpressed during heart failure. A GWAS study identified an association between esophageal squamous cell carcinoma and a common DNA variant in the PLCE1 gene in East Asians. Two isoforms of the human protein are produced by alternative splicing. Isoform 1 is broadly expressed and only absent in peripheral blood leukocytes. Isoform 2 is specifically expressed in placenta, lung and spleen. Isoform 1 and isoform 2 associate with Golgi membranes.

Protein type: Carbohydrate Metabolism - inositol phosphate; GAPs, Ras; EC 3.1.4.11; GAPs; Phospholipase

Cellular Component: cytoplasm; cytosol; Golgi apparatus; membrane; plasma membrane

Molecular Function: enzyme binding; guanyl-nucleotide exchange factor activity; hydrolase activity; phosphoinositide phospholipase C activity; phospholipase C activity; phosphoric diester hydrolase activity; protein binding; Ras GTPase binding; signal transducer activity

Biological Process: activation of MAPK activity; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); glomerulus development; lipid catabolic process; lipid metabolic process; Ras protein signal transduction; regulation of G-protein coupled receptor protein signaling pathway; regulation of protein kinase activity; regulation of Ras protein signal transduction; signal transduction; small GTPase mediated signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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