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PLCG1 elisa kit :: Human 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 ELISA Kit

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Catalog # MBS2885880
Unit / Price
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  48-Strip-Wells  /  $355 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,030 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,700 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 (PLCG1), ELISA Kit

Full Product Name

Human 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 ELISA Kit

Product Synonym Names
1-phosphatidylinositol-4; 5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
172420
3D Structure
ModBase 3D Structure for P19174
Species Reactivity
Specificity
This assay recognizes recombinant and natural human 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1. No significant cross-reactivity or interference was observed.
Assay Type
Sandwich
Detection Range
0.156-10 ng/mL
Sensitivity
0.1ng/mL
Intra-Assay CV
<=5.7%
Inter-Assay CV
<=8.3%
Recovery
101%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PLCG1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PLCG1purchase
MBS2885880 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 (PLCG1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLCG1. The ELISA analytical biochemical technique of the MBS2885880 kit is based on PLCG1 antibody-PLCG1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLCG1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLCG1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PLCG1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PLCG1 elisa kit
PLCG1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2885880. Click to request current manual
NCBI/Uniprot data below describe general gene information for PLCG1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
148,660 Da
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform a
NCBI Official Synonym Full Names
phospholipase C gamma 1
NCBI Official Symbol
PLCG1  [Similar Products]
NCBI Official Synonym Symbols
PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
  [Similar Products]
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
UniProt Synonym Protein Names
PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1; PLC-gamma-1
UniProt Gene Name
PLCG1  [Similar Products]
UniProt Synonym Gene Names
PLC1; PLC-II; PLC-gamma-1  [Similar Products]
UniProt Entry Name
PLCG1_HUMAN
NCBI Summary for PLCG1
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for PLCG1
PLCG1: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors.

Protein type: EC 3.1.4.11; Phospholipase; Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: cell projection; cytoplasm; cytosol; intercellular junction; lamellipodium; plasma membrane; ruffle; signalosome

Molecular Function: calcium ion binding; glutamate receptor binding; neurotrophin TRKA receptor binding; phosphoinositide phospholipase C activity; phospholipase C activity; protein binding; protein kinase binding; receptor signaling protein activity; receptor tyrosine kinase binding

Biological Process: activation of MAPKK activity; axon guidance; blood coagulation; calcium-mediated signaling; cell migration; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; in utero embryonic development; innate immune response; inositol phosphate metabolic process; leukocyte migration; nerve growth factor receptor signaling pathway; phospholipase C activation; phospholipid catabolic process; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of release of sequestered calcium ion into cytosol; signal transduction; T cell receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral reproduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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