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PLCgamma1 elisa kit :: Human PLCgamma1 ELISA Kit

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Catalog # MBS2505694
Unit / Price
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

PLCgamma1, ELISA Kit

Popular Item
Full Product Name

Human PLCgamma1 (Phospholipase C Gamma 1) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P19174
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of Human PLCgamma1. No significant cross-reactivity or interference between Human PLCgamma1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PLCgamma1 and all the analogues, therefore, cross reaction may still exist.
Serum, Plasma, Biological Fluids
Assay Type
Detection Range
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PLCgamma1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PLCgamma1purchase
MBS2505694 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PLCgamma1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLCgamma1. The ELISA analytical biochemical technique of the MBS2505694 kit is based on PLCgamma1 antibody-PLCgamma1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLCgamma1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLCgamma1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PLCgamma1 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PLCgamma1 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PLCgamma1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PLCgamma1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLCgamma1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PLCgamma1. You can calculate the concentration of Human PLCgamma1 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PLCgamma1 elisa kit
PLCgamma1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2505694. Click to request current manual
NCBI/Uniprot data below describe general gene information for PLCgamma1. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
148,660 Da
NCBI Official Full Name
Phospholipase C, gamma 1
NCBI Official Synonym Full Names
phospholipase C, gamma 1
NCBI Official Symbol
PLCG1  [Similar Products]
NCBI Official Synonym Symbols
PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
  [Similar Products]
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; PLC-gamma-1; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; phosphoinositide phospholipase C-gamma-1; phospholipase C, gamma 1 (formerly subtype 148); phospholipase C-148; phospholipase C-II; triphosphoinositide phosphodiesterase
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
UniProt Synonym Protein Names
PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1; PLC-gamma-1
UniProt Gene Name
PLCG1  [Similar Products]
UniProt Synonym Gene Names
PLC1; PLC-II; PLC-gamma-1  [Similar Products]
UniProt Entry Name
NCBI Summary for PLCgamma1
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for PLCgamma1
PLCG1: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors.

Protein type: Phospholipase; Motility/polarity/chemotaxis; EC; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: signalosome; ruffle; cell projection; lamellipodium; cytoplasm; plasma membrane; intercellular junction; cytosol

Molecular Function: protein binding; glutamate receptor binding; neurotrophin TRKA receptor binding; receptor signaling protein activity; calcium ion binding; receptor tyrosine kinase binding; phospholipase C activity; phosphoinositide phospholipase C activity; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; axon guidance; cell migration; fibroblast growth factor receptor signaling pathway; inositol phosphate metabolic process; nerve growth factor receptor signaling pathway; viral reproduction; activation of MAPKK activity; in utero embryonic development; phospholipid catabolic process; cytokine and chemokine mediated signaling pathway; positive regulation of blood vessel endothelial cell migration; calcium-mediated signaling; signal transduction; T cell receptor signaling pathway; positive regulation of angiogenesis; phospholipase C activation; innate immune response; positive regulation of release of sequestered calcium ion into cytosol; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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