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PLCL1 elisa kit :: Human phospholipase C-like 1 ELISA Kit

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Catalog # MBS922790
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Typical Testing Data/Standard Curve (for reference only)
Product Name

phospholipase C-like 1 (PLCL1), ELISA Kit

Popular Item
Full Product Name

Human Inactive phospholipase C-like protein 1, PLCL1 ELISA Kit

Product Synonym Names
Human Inactive phospholipase C-like protein 1 (PLCL1) ELISA kit; MGC126580; MGC138190; PLCE; PLCL; PLDL1; PRIP; phospholipase C related; but catalytically inactive protein; phospholipase C; epsilon; phospholipase C-like 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS922790 Testing Data
OMIM
600597
3D Structure
ModBase 3D Structure for Q15111
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human PLCL1. No significant cross-reactivity or interference between human PLCL1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
46.88 pg/ml -3000 pg/ml.
Sensitivity
The minimum detectable dose of human PLCL1 is typically less than 11.7 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PLCL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PLCL1purchase
MBS922790 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the phospholipase C-like 1 (PLCL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLCL1. The ELISA analytical biochemical technique of the MBS922790 kit is based on PLCL1 antibody-PLCL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLCL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLCL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PLCL1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLCL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLCL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLCL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLCL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of PLCL1 elisa kit
PLCL1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS922790. Click to request current manual
NCBI/Uniprot data below describe general gene information for PLCL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
122,728 Da
NCBI Official Full Name
inactive phospholipase C-like protein 1
NCBI Official Synonym Full Names
phospholipase C-like 1
NCBI Official Symbol
PLCL1  [Similar Products]
NCBI Official Synonym Symbols
PLCE; PLCL; PRIP; PLDL1; PPP1R127
  [Similar Products]
NCBI Protein Information
inactive phospholipase C-like protein 1; PLC-L1; phospholipase C, epsilon; phospholipase C-deleted in lung carcinoma; protein phosphatase 1, regulatory subunit 127; phospholipase C-related but catalytically inactive protein; phospholipase C related, but catalytically inactive protein
UniProt Protein Name
Inactive phospholipase C-like protein 1
UniProt Synonym Protein Names
Phospholipase C-deleted in lung carcinoma; Phospholipase C-related but catalytically inactive protein; PRIP
UniProt Gene Name
PLCL1  [Similar Products]
UniProt Synonym Gene Names
PLC-L1; PRIP  [Similar Products]
UniProt Entry Name
PLCL1_HUMAN
UniProt Comments for PLCL1
PLCL1: a protein closely related to phospholipase C-d1 (PLC-d1) but catalytically inactive. First identified as a novel inositol 1,4,5-trisphosphate binding protein. Has a number of binding partners including protein phosphatase (PP1 and 2A), GABA(A) receptor associated protein, and the beta subunits of GABA(A) receptors, in addition to inositol 1,4,5-trisphosphate. Plays an important role in controlling the dynamics of GABA(A) receptor phosphorylation through PP1 binding and, therefore, the efficacy of synaptic inhibition mediated by these receptors.

Protein type: Phospholipase

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: cytoplasm; plasma membrane

Molecular Function: signal transducer activity; GABA receptor binding; phosphoinositide phospholipase C activity; phospholipase C activity

Biological Process: regulation of peptidyl-serine phosphorylation; lipid metabolic process; regulation of synaptic transmission, GABAergic; gamma-aminobutyric acid signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with PLCL1 elisa kit
Diseases associated with PLCL1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1 publications with PLCL1 and Inflammation
Organs/Tissues associated with PLCL1 elisa kit
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