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PLCZ1 recombinant protein :: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 Recombinant Protein

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Catalog # MBS1451131
Unit / Price
Scan QR to view Datasheet
  0.01 mg (E-Coli)  /  $160 +1 FREE 8GB USB
  0.05 mg (E-Coli)  /  $200 +1 FREE 8GB USB
  0.1 mg (E-Coli)  /  $295 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $480 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $790 +1 FREE 8GB USB
  1 mg (E-Coli)  /  $1,215 +1 FREE 8GB USB
SDS-Page
Product Name

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (PLCZ1), Recombinant Protein

Popular Item
Full Product Name

Recombinant Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Product Synonym Names
Phosphoinositide phospholipase C-zeta-1; Phospholipase C-zeta-11
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-415aa; Full length of isoform 2
Sequence
MEMRWFLSKI QDDFRGGKIN LEKTQRLLEK LDIRCSYIHV KQIFKTSDYP VVLSLENHCS TAQQEVMADN LQATFGESLL SDMLDDFPDT LPSPEALKFK ILVKNKKIGT LKETHERKGS DKRGDNQDKE TGVKKLPGVM LFKKKKTRKL KIALALSDLV IYTKAEKFKS FQHSRLYQQF NENNSIGETQ ARKLSKLRVH EFIFHTRKFI TRIYPKATRA DSSNFNPQEF WNIGCQMVAL NFQTPGLPMD LQNGKFLDNG GSGYILKPHF LRESKSYFNP SNIKEGMPIT LTIRLISGIQ LPLTHSSSNK GDSLVIIEVF GVPNDQMKQQ TRVIKKNAFS PRWNETFTFI IHVPELALIR FVVEGQGLIA GNEFLGQYTL PLLCMNKGYR RIPLFSRMGE SLEPASLFVY VWYVR
OMIM
608075
3D Structure
ModBase 3D Structure for Q86YW0
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of PLCZ1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PLCZ1 recombinant protein
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.
Product Categories/Family for PLCZ1 recombinant protein

PLCZ1 recombinant protein SDS-Page image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for PLCZ1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
63.7kD
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
NCBI Official Synonym Full Names
phospholipase C zeta 1
NCBI Official Symbol
PLCZ1  [Similar Products]
NCBI Official Synonym Symbols
PLCzeta; NYD-SP27; PLC-zeta-1
  [Similar Products]
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
UniProt Synonym Protein Names
Phosphoinositide phospholipase C-zeta-1; Phospholipase C-zeta-1
UniProt Gene Name
PLCZ1  [Similar Products]
UniProt Synonym Gene Names
PLC-zeta-1  [Similar Products]
UniProt Entry Name
PLCZ1_HUMAN
NCBI Summary for PLCZ1
The protein encoded by this gene is a member of the phosphoinositide-specific phospholipase C family. Members in this family, classified into six isotypes that are tissue- and organ-specific, hydrolyze phosphatidylinositol 4,5-bisphosphate just before the phosphate group to yield diacylglycerol and inositol 1,4,5-trisphosphate. This protein localizes to the acrosome in spermatozoa and elicits Ca(2+) oscillations and egg activation during fertilization that leads to early embryonic development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
UniProt Comments for PLCZ1
PLCZ1: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)- dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg- activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.11; Phospholipase; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: cytosol; nucleus; perinuclear region of cytoplasm

Molecular Function: calcium ion binding; phosphoinositide phospholipase C activity; signal transducer activity

Biological Process: calcium ion transport; egg activation; inositol phosphate metabolic process; lipid catabolic process; multicellular organismal development
Product References and Citations for PLCZ1 recombinant protein
Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytes.Cox L.J., Larman M.G., Saunders C.M., Hashimoto K., Swann K., Lai F.A.Reproduction 124:611-623(2002) Rescue of defective pancreatic secretion in cystic-fibrosis cells by suppression of a novel isoform of phospholipase C.Zhu H., Zhu J.X., Lo P.S., Li J., Leung K.M., Rowlands D.K., Tsang L.L., Yu M.K., Jiang J.L., Lam S.Y., Chung Y.W., Zhou Z., Sha J., Chang Chan H.Lancet 362:2059-2065(2003) Complete sequencing and characterization of 21,243 full-length human cDNAs.Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.Nat. Genet. 36:40-45(2004) Phospholipase Czeta causes Ca2+ oscillations and parthenogenetic activation of human oocytes.Rogers N.T., Hobson E., Pickering S., Lai F.A., Braude P., Swann K.Reproduction 128:697-702(2004)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Products associated withPLCZ1 recombinant protein
 Reference Product  PubMed Publications
 ITPR1 recombinant protein  >2 publications with PLCZ1 and ITPR1
Organs/Tissues associated with PLCZ1 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >8 publications with PLCZ1 and Testis
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