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PLD1 elisa kit :: Human phospholipase D1, phosphatidylcholine-specific ELISA Kit

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Catalog # MBS914961
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Typical Testing Data/Standard Curve (for reference only)
Product Name

phospholipase D1, phosphatidylcholine-specific (PLD1), ELISA Kit

Popular Item
Full Product Name

Human Phospholipase D1, PLD1 ELISA Kit

Product Synonym Names
Human Phospholipase D1 (PLD1) ELISA kit; choline phosphatase 1; phospholipase D1; phospholipase D1; phophatidylcholine-specific; phospholipase D1; phosphatidylcholine-specific
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS914961 Testing Data
OMIM
602382
3D Structure
ModBase 3D Structure for Q13393
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human PLD1. No significant cross-reactivity or interference between human PLD1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
25 pg/ml -1600 pg/ml.
Sensitivity
The minimum detectable dose of human PLD1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PLD1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PLD1purchase
MBS914961 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the phospholipase D1, phosphatidylcholine-specific (PLD1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLD1. The ELISA analytical biochemical technique of the MBS914961 kit is based on PLD1 antibody-PLD1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLD1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLD1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PLD1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of PLD1 elisa kit
PLD1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS914961. Click to request current manual
NCBI/Uniprot data below describe general gene information for PLD1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
124,184 Da
NCBI Official Full Name
phospholipase D1 isoform a
NCBI Official Synonym Full Names
phospholipase D1, phosphatidylcholine-specific
NCBI Official Symbol
NCBI Protein Information
phospholipase D1; choline phosphatase 1; phosphatidylcholine-hydrolyzing phospholipase D1
UniProt Protein Name
Phospholipase D1
UniProt Synonym Protein Names
Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PLD 1; hPLD1  [Similar Products]
UniProt Entry Name
PLD1_HUMAN
NCBI Summary for PLD1
This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011]
UniProt Comments for PLD1
PLD1: a phosphatidylcholine-hydrolyzing phospholipase. Activated by ADP-ribosylated Rho and protein kinase C. Implicated as a critical step in numerous cellular pathways including membrane trafficking and the regulation polymorphonuclear leukocyte degranulation and oxidant production. May be involved in the regulation of perinuclear intravesicular membrane traffic. Four splice-variant isoforms have been described.

Protein type: Lipid Metabolism - glycerophospholipid; Phospholipase; Motility/polarity/chemotaxis; Lipid Metabolism - ether lipid; EC 3.1.4.4

Chromosomal Location of Human Ortholog: 3q26

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; late endosome membrane; endocytic vesicle; perinuclear region of cytoplasm; lysosomal membrane; apical plasma membrane; endosome

Molecular Function: protein binding; phospholipase D activity; phosphoinositide binding

Biological Process: defense response to Gram-positive bacterium; phosphatidylglycerol biosynthetic process; small GTPase mediated signal transduction; phospholipid metabolic process; glycerophospholipid biosynthetic process; Ras protein signal transduction; phosphatidic acid biosynthetic process; chemotaxis; lipid catabolic process; regulation of microvillus biogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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