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PNPLA2 elisa kit :: Rat patatin-like phospholipase domain containing 2 ELISA Kit

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Catalog # MBS702087
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
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  96-Strip-Wells  /  $625 +1 FREE 8GB USB
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PNPLA2 elisa kit
Product Name

patatin-like phospholipase domain containing 2 (PNPLA2), ELISA Kit

Full Product Name

Rat Adipose Triglyceride Lipase, ATGL ELISA Kit

Product Synonym Names
Rat Adipose Triglyceride Lipase; ATGL ELISA Kit; 1110001C14Rik; ATGL; DESNUTRIN; DKFZp667M109; FP17548; PEDF-R; TTS-2.2; TTS2; adipose triglyceride lipase; pigment epithelium-derived factor; transport-secretion protein 2.2; triglyceride hydrolase; patatin-like phospholipase domain containing 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P0C548
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of rat ATGL. No significant cross-reactivity or interference between rat ATGL and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.312 ng/ml-20 ng/ml.
Sensitivity
The minimum detectable dose of rat ATGL is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PNPLA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PNPLA2purchase
MBS702087 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the patatin-like phospholipase domain containing 2 (PNPLA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PNPLA2. The ELISA analytical biochemical technique of the MBS702087 kit is based on PNPLA2 antibody-PNPLA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PNPLA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PNPLA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PNPLA2 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATGL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATGL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATGL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATGL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS702087. Click to request current manual
NCBI/Uniprot data below describe general gene information for PNPLA2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
52,567 Da
NCBI Official Full Name
patatin-like phospholipase domain-containing protein 2
NCBI Official Synonym Full Names
patatin-like phospholipase domain containing 2
NCBI Official Symbol
Pnpla2  [Similar Products]
NCBI Official Synonym Symbols
RGD1309044
  [Similar Products]
NCBI Protein Information
patatin-like phospholipase domain-containing protein 2; adipose triglyceride lipase
UniProt Protein Name
Patatin-like phospholipase domain-containing protein 2
UniProt Synonym Protein Names
Adipose triglyceride lipase
UniProt Gene Name
Pnpla2  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
PLPL2_RAT
UniProt Comments for PNPLA2
Function: Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion

Catalytic activity: Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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