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PODXL elisa kit :: Human Podocalyxin ELISA Kit

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Catalog # MBS765311
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Podocalyxin (PODXL), ELISA Kit

Popular Item
Also Known As

Human Podocalyxin ELISA Kit

Product Synonym Names
PCX (Podocalyxin)/PODXL/PCLP/PCLP1/Podocalyxin-like protein 1/PC/PCLP-1/GCTM-2 antigen/Gp200
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS765311 COA
MBS765311 QC Report
Sequence Length
485
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of PODXL. No significant cross-reactivity or interference between PODXL and analogues was observed.
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PODXL were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PODXL were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PODXL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPODXLpurchase
MBS765311 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Podocalyxin (PODXL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PODXL. The ELISA analytical biochemical technique of the MBS765311 kit is based on PODXL antibody-PODXL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PODXL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PODXL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PODXL elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PODXL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PODXL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PODXL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PODXL can be calculated.

Typical Testing Data/Standard Curve (for reference only) of PODXL elisa kit
PODXL elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS765311. Click to request current manual
NCBI/Uniprot data below describe general gene information for PODXL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,658 Da
NCBI Official Full Name
podocalyxin
NCBI Official Synonym Full Names
podocalyxin-like
NCBI Official Symbol
Podxl  [Similar Products]
NCBI Official Synonym Symbols
PC; PCLP-1; podocalyxin
  [Similar Products]
NCBI Protein Information
podocalyxin
UniProt Protein Name
Podocalyxin
UniProt Synonym Protein Names
Podocalyxin-like protein 1; PC; PCLP-1
Protein Family
UniProt Gene Name
Podxl  [Similar Products]
UniProt Synonym Gene Names
Pclp1; PC; PCLP-1  [Similar Products]
UniProt Entry Name
PODXL_RAT
NCBI Summary for PODXL
inhibits cell-cell adhesion; may play a role in maintaining filtration in the glomerular epithelium [RGD, Feb 2006]
UniProt Comments for PODXL
PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: apical plasma membrane; cell projection; cytoplasm; extracellular space; filopodium; integral to membrane; intracellular membrane-bound organelle; lamellipodium; lipid raft; microvillus membrane; plasma membrane; ruffle

Molecular Function: protein binding

Biological Process: cell adhesion; cell migration; glomerular filtration; leukocyte migration; negative regulation of cell adhesion; negative regulation of cell-cell adhesion; positive regulation of cell migration; positive regulation of cell-cell adhesion mediated by integrin; regulation of cell-cell adhesion; regulation of microvillus biogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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