• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-POLH antibody :: Rabbit POLH Antibody

Scan QR to view Datasheet
Catalog # MBS8501893
Unit / Price
  0.1 mL  /  $310 +1 FREE 8GB USB
Testing Data
Product Name

POLH, Antibody

Popular Item
Also Known As

POLH Antibody

Product Synonym Names
FLJ16395; FLJ21978; RAD30; RAD30A; XP-V; XPV
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
414
OMIM
278750
3D Structure
ModBase 3D Structure for Q9Y253
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity purification
Immunogen
Recombinant protein of human POLH
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-POLH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-POLH antibody
This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. [provided by RefSeq, Jul 2008]
Applications Tested/Suitable for anti-POLH antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-POLH antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Testing Data of anti-POLH antibody
Western blot analysis of extracts of A431cell lines, using POLH antibody.
anti-POLH antibody Testing Data image
NCBI/Uniprot data below describe general gene information for POLH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
78kDa
NCBI Official Full Name
DNA polymerase eta isoform 3
NCBI Official Synonym Full Names
DNA polymerase eta
NCBI Official Symbol
NCBI Official Synonym Symbols
XPV; XP-V; RAD30; RAD30A
  [Similar Products]
NCBI Protein Information
DNA polymerase eta
UniProt Protein Name
DNA polymerase eta
UniProt Synonym Protein Names
RAD30 homolog A; Xeroderma pigmentosum variant type protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
RAD30; RAD30A; XPV  [Similar Products]
NCBI Summary for POLH
This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Comments for POLH
DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed:10385124, PubMed:11743006, PubMed:24449906). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed:24449906). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed:10385124, PubMed:11743006). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes (PubMed:11376341, PubMed:14734526). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (PubMed:14630940). Targets POLI to replication foci (PubMed:12606586).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000