• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-POLR2A antibody :: Rabbit POLR2A (Ab-1619) Antibody

Scan QR to view Datasheet
Catalog # MBS002301
Unit / Price
Scan QR to view Datasheet
  0.1 mL  /  $440 +1 FREE 8GB USB
  0.2 mL  /  $715 +1 FREE 8GB USB
Product Name

POLR2A (Ab-1619), Antibody

Popular Item
Full Product Name

Rabbit POLR2A (Ab-1619) Antibody

Product Synonym Names
RPO2; RPOL2,POLR2 ALPHA-AMANITIN RESISTANCE,POLYMERASE II RNA, SUBUNIT A,RNA POLYMERASE II, 220-KD SUBUNIT,RNA POLYMERASE II, LARGE SUBUNIT,RPB1, S. CEREVISIAE, HOMOLOG OF polymerase (RNA) II (DNA directed) polypeptide A
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 17; NC_000017.10 (7387698..7417935). Location: 17p13.1
OMIM
180660
3D Structure
ModBase 3D Structure for P24928
Host
Rabbit
Species Reactivity
Human, mouse, rat
Specificity
POLR2A (Ab-1619) antibody detects endogenous levels of total POLR2A protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human POLR2A around the phosphorylation site of serine 1619 (P-T-S-P-S).
Preparation and Storage
Store at -20 degree C/1 year
Other Notes
Small volumes of anti-POLR2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-POLR2A antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-POLR2A antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000

Immunohistochemical of anti-POLR2A antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using POLR2A (Ab-1619) antibody.
anti-POLR2A antibody Immunohistochemical image
Western blot of anti-POLR2A antibody
Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30min), using POLR2A (Ab-1619) antibody
anti-POLR2A antibody Western blot image
Immunofluorescence of anti-POLR2A antibody
Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using POLR2A (Ab-1619) antibody.
anti-POLR2A antibody Immunofluorescence image
NCBI/Uniprot data below describe general gene information for POLR2A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
217,176 Da
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB1
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
NCBI Official Symbol
POLR2A  [Similar Products]
NCBI Official Synonym Symbols
RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
  [Similar Products]
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; RNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB1
UniProt Synonym Protein Names
DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1
UniProt Gene Name
POLR2A  [Similar Products]
UniProt Synonym Gene Names
POLR2; RNA polymerase II subunit B1  [Similar Products]
UniProt Entry Name
RPB1_HUMAN
NCBI Summary for POLR2A
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
UniProt Comments for POLR2A
POLR2A: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA- dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. The phosphorylated C-terminal domain interacts with FNBP3 and SYNCRIP. Interacts with SAFB/SAFB1. Interacts with CCNL1 and MYO1C. Interacts with CCNL2 and SFRS19. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with PAF1. Interacts (via C-terminus) with FTSJD2, CTDSP1 and SCAF8. Interacts via the phosphorylated C-terminal domain with WDR82 and with SETD1A and SETD1B only in the presence of WDR82. Interacts with ATF7IP. When phosphorylated at 'Ser-5', interacts with MEN1; the unphosphorylated form, or phosphorylated at 'Ser-2' does not interact. Interacts with DDX5. Belongs to the RNA polymerase beta' chain family.

Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transferase; EC 2.7.7.6; Transcription initiation complex; EC 2.7.7.48

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleoplasm; nucleolus; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function: protein binding; RNA-directed RNA polymerase activity; DNA binding; DNA-directed RNA polymerase activity; ubiquitin protein ligase binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; somatic stem cell maintenance; positive regulation of viral transcription; RNA splicing; DNA repair; positive regulation of RNA splicing; nuclear mRNA splicing, via spliceosome; mRNA capping; regulation of transcription, DNA-dependent; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000