View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List Goat 4-Hydroxynonenal Polyclonal Antibody Catalog # MBS316152 Unit / Price DISCONTINUEDYou will be redirected to our replacement product Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name 4-Hydroxynonenal, Polyclonal Antibody Popular Item Full Product Name Goat anti 4-Hydroxynonenal Product Synonym Names Goat Antibody to 4-Hydroxynonenal Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host Goat Specificity 4-Hydroxynonenal4-Hydroxynonenal. Reactive with 4-HNE conjugated BSA. Has minimal cross reactivity with other aldehyde-BSA conjugates. Form/Format Neat, Liquid Concentration Not Determined. (lot specific) Immunogen 4-Hydroxynonenal conjugated Bovine Serum Albumin (Catalog #MBS318058) Buffer Not applicable Preservative 0.1% Sodium azide Important Note Centrifuge before opening to ensure complete recovery of vial contents. Warnings This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. Preparation and Storage Short-term store at 2 to 8 degree C. Long term store at -20 degree C. ISO Certification Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory. Other Notes Small volumes of anti-4-Hydroxynonenal antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Product Categories/Family for anti-4-Hydroxynonenal antibody Polyclonal Antibodies for Neuroscience Applications Tested/Suitable for anti-4-Hydroxynonenal antibody EIA/ELISA, Immunohistochemistry Application Notes for anti-4-Hydroxynonenal antibody ELISA using immunizing peptide: >1:10,000. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Immunohistochemistry of anti-4-Hydroxynonenal antibody Customer reported this item can be applied to mouse tissue in IHC paraffin. The photos below,which appear positive for fatty liver and negative for another normal one.1. citrate buffer pH 6 100 degree C for 20 min. (RE7113 Leica Novocastra)2. primary Ab. 200 X for 30 min at Room Temperature3. rabbit x goat conjugate HRP 500 X for 20 min at RT. Rabbit anti-Goat IgG antibody HRP Conjugated4. anti Rabbit polymer HRP(RTU) for 10min at RT (RE7112 Leica Novocastra)5. DAB for 3 min. (RE7190K Leica Novocastra) TOP Product References and Citations for anti-4-Hydroxynonenal antibody • BoBoon, P.J.M., et al., (1999), Tox. Appl. Pharmacol., 159, 214-223.on, P.J.M., et al., (1999), Tox. Appl. Pharmacol., 159, 214-223. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP