• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Sheep 5-METHYLCYTOSINE Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS223530
Unit / Price
Scan QR to view Datasheet
  0.1 mg  /  $300 +1 FREE 8GB USB
anti-5-METHYLCYTOSINE antibody
Product Name

5-METHYLCYTOSINE, Polyclonal Antibody

Popular Item
Full Product Name

SHEEP ANTI 5-METHYLCYTOSINE:Preservative Free

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Form/Format
Preservative Free
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Buffer Solution
Phosphate buffered saline
Antiserum Preparation
Antiserum to 5-MeC was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species
Broad
Preparation and Storage
Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-5-METHYLCYTOSINE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-5-METHYLCYTOSINE antibody
Sheep Anti 5-Methylcytosine (5-MeC) recognizes the methylated form of cytosine (C), an epigenetic modification catalyzed by DNA methyltransferases resulting in the attachment of a methyl group to carbon 5. This modification alters the structure of cytosine but not its base-pairing properties. The timing and function of cytosine methylation varies amongst species with roles in such processing events as transcription and recombination. For example, in mammals, methylation occuring during early development and emrbyogensis functions in X chromosome inactivation and gene imprinting, wherease in plants, DNA methylation occurs later and affects genomic stability through silencing of transposable elements (He et al. 2011).The deamination of 5-MeC results in the formation of thymine (T) instead of uracil. Since thymine is not recognized by DNA repair enzymes, cytosine to thymine conversions can cause transition mutations. The most frequent mutations in human genetic diseases are those involving C to T transitions occurring at CG sites (Morgan et al. 2004). Another type of mutation involving 5-MeC involves the formation of pyrimidine dimers. Upon exposure to sunlight, pyrimidine dimers form preferentially at dipyrimidine sequences containing 5-MeC, a mutation frequently seen in human skin cancers (Pfeifer et al. 2005). Sheep Anti-5-MeC is reported as suitable for use in immunocytochemistry.
Applications Tested/Suitable for anti-5-METHYLCYTOSINE antibody
Immunohistology Frozen*, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-5-METHYLCYTOSINE antibody
Immunohistology - Frozen: Minimum Dilution: 10ug/ml; Application Note: Using a fixation step in cold methanol for 30 minutes followed by immersion in 0.007 M NaOH for 10-15 seconds allows staining with the simultaneous detection of nuclear cytoplasmic and membrane components, as well as preservation of morphological detail.
Immunoprecipitation: Minimum Dilution: 25; Maximum Dilution: 100ug/ml;
Immunohistology - Paraffin: Minimum Dilution: 10ug/ml; Application Note: * Using a fixation step in cold methanol for 30 minutes followed by immersion in 0.007 M NaOH for 10-15 seconds allows staining with the simultaneous detection of nuclear cytoplasmic and membrane components, as well as preservation of morphological detail.
Western Blotting: Minimum Dilution: 1:1000
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000