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anti-A2M antibody :: Goat alpha2-Macroglobulin Polyclonal Antibody

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Catalog # MBS512011
Unit / Price
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  0.5 mg  /  $345 +1 FREE 8GB USB
anti-A2M antibody
Product Name

alpha2-Macroglobulin (A2M), Polyclonal Antibody

Full Product Name

Goat anti-human alpha2Macroglobulin (alpha2M), Affinity-purified IgG

Product Synonym Names
alpha2-Macroglobulin, human
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 12; NC_000012.11 (9220304..9268558, complement). Location: 12p13.31
OMIM
103950
3D Structure
ModBase 3D Structure for P01023
Clonality
Polyclonal
Host
Goat
Specificity
This antibody is specific for alpha2macroglobulin as demonstrated by immunoelectrophoresis and ELISA.
Purity/Purification
Affinity purified IgG.
Vial containing ml of IgG purified by affinitychromatography on immobilized alpha2M. Total protein is 0.5 mg.
Form/Format
Affinity-purified IgG (APIgG), clear liquid.
Concentration
APIgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4. (lot specific)
Immunogen
Human alpha2macroglobulin purified from plasma.
Buffer
10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.
Neutralizing Activity
Not determined
Preparation and Storage
Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.
Other Notes
Small volumes of anti-A2M antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-A2M antibody
alpha2Macroglobulin (alpha2M) is a large proteinase inhibitor molecule of 718,000 daltons, consisting of 4 identical subunits of 185,000 each. Produced in hepatocytes and macrophages, plasma concentrations of alpha2M are typically 2 uM in adults, and as high as 6 uM in childhood. alpha2M has the ability to inhibit most enzymes from the serine, metallo, cysteine and aspartate subclasses. It is not a member of the SERPIN family of inhibitors but belongs to a class of proteins that include pregnancy zone protein (PZP) and the complement proteins C3, C4 and C5. These proteins contain regions of conserved sequence as well as one or more internal beta-cysteinyl-gamma- glutamyl thiolester bonds, which in the case of alpha2M are susceptible to cleavage by enzymes or by nucleophilic compounds such as methylamine or ammonium ions. Although the precise nature of the interactions is as yet unknown, it is generally thought that cleavage of a bait region within the alpha2M molecule by an enzyme leads to a conformational change, which then traps and/or covalently binds the enzyme1,2. The active site of the trapped enzyme is usually still intact and able to cleave small substrates, but is inaccessible to larger natural substrates. The conformational change induced also exposes receptor-binding regions within the molecule, which may be important in the clearance of alpha2M-enzyme complexes from the circulation. It is thought that the main role of alpha2M in vivo is that of a "backup" inhibitor and scavenger of proteinases in blood and in tissues3,4, but it has also been reported to participate in other physiological processes, including regulation of immune function1,2.
Application Notes for anti-A2M antibody
Suitable for use as a source of enriched antibodies to alpha2M.
NCBI/Uniprot data below describe general gene information for A2M. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
163,291 Da[Similar Products]
NCBI Official Full Name
Alpha-2-macroglobulin
NCBI Official Synonym Full Names
alpha-2-macroglobulin
NCBI Official Symbol
NCBI Official Synonym Symbols
A2MD; CPAMD5; FWP007; S863-7; DKFZp779B086
  [Similar Products]
NCBI Protein Information
alpha-2-macroglobulin; alpha-2-M; OTTHUMP00000196829; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
UniProt Protein Name
Alpha-2-macroglobulin
UniProt Synonym Protein Names
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CPAMD5  [Similar Products]
UniProt Entry Name
A2MG_HUMAN
NCBI Summary for A2M
Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq]
UniProt Comments for A2M
Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Subunit structure: Homotetramer; disulfide-linked. Ref.8 Ref.10

Subcellular location: Secreted Ref.8.

Tissue specificity: Secreted in plasma. Ref.8

Developmental stage: Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.

Sequence similarities: Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. [View classification]

Sequence caution: The sequence AAT02228.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAD92851.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
Product References and Citations for anti-A2M antibody
1. Salvesen G, Pizzo SV; Proteinase Inhibitors: alpha-Macroglobulins, Serpins and Kunins; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 241-258, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
2. Barrett J; alpha2Macroglobulin; Methods in Enzymology, 80, pp 737-754, 1981 Larsson LJ, Neuenschwander DE, Strickland DK; Reaction of Proteinases with alpha2Macroglobulin: Evidence for Alternate Reaction Pathways in the Inhibition of Trypsin; Biochemistry 28, pp 7636-7643, 1989.
3. Schmidt B, Mitchell L, Ofosu FA, Andrew M; alpha2Macroglobulin is an Important Progressive Inhibitor of Thrombin in Neonatal and Infant Plasma; Thromb Haemostas 62, pp 1074-1077, 1989.
4. Hoogendoorn H, Toh CH, Nesheim ME, Giles AR; alpha2Macroglobulin Binds and Inhibits Activated Protein C; Blood 78, pp2283-2290, 1991.

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