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anti-APOH antibody :: Rabbit beta-2 glycoprotein 1 Polyclonal Antibody

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Catalog # MBS573737
Unit / Price
  1 mL  /  $570 +1 FREE 8GB USB
anti-APOH antibody
Product Name

beta-2 glycoprotein 1 (APOH), Polyclonal Antibody

Popular Item
Also Known As

Rabbit anti Human beta-2 glycoprotein 1

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 17; NC_000017.10 (64208147..64225556, complement). Location: 17q24.2
OMIM
138700
3D Structure
ModBase 3D Structure for P02749
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Specificity
In bidimensional immunoelectrophoresis a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of human serum or plasma.
Form/Format
Delipidated, heat inactivated, lyophilized, stable whole serum.
Biological origin
Rabbit
Preservative
No preservative added.
Immunogen
Beta-2 glycoprotein 1 has a molecular weight of 40,000. The mean concentration in adult serum is about 200 I-1g/ml. Freund's complete adjuvant is used in the first step of the immunization procedure.
Adsorption
Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Protein concentration
Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added.
Antibody titre
Measured by quantitative precipitin analysis. The amount of beta2 glycoprotein 1 precipitated by 1ml antiserum is approximately 0.2 mg. Precipitin titre not less than 1:64 when tested against normal human serum in agar-block immunodiffusion titration.
Directions for use
In immunoelectrophoresis use 2 1-11 human plasma or equivalent against 120 1-11 antiserum. In double radial immunodiffusion use a rosette arrangement with 10 ul antiserum in 3 mm diameter center well and 2 ul plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In electroimmunodiffusion use 1 percent antiserum in the gel.
Preparation and Storage
Storage / shelf life :
Lyophilized at +4 degree C = at least 10 years
reconstituted at or below -20 degree C = 3-5 years
reconstituted at +4 degree C = 7 days

Handling: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 degree C; prolonged
storage at or below -20 degree C . Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 degree C, not refrozen, and preferably used the same day.

Caution: This antiserum should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro research purposes only.
Other Notes
Small volumes of anti-APOH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-APOH antibody
In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell) to identify the presence of beta-2 glycoprotein 1 or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques as ELISA and Western blotting if proper controls are included.
Product Categories/Family for anti-APOH antibody
Applications Tested/Suitable for anti-APOH antibody
Immunoprecipitation (IP)
NCBI/Uniprot data below describe general gene information for APOH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,298 Da[Similar Products]
NCBI Official Full Name
beta-2-glycoprotein 1
NCBI Official Synonym Full Names
apolipoprotein H (beta-2-glycoprotein I)
NCBI Official Symbol
NCBI Official Synonym Symbols
BG; B2G1; B2GP1
  [Similar Products]
NCBI Protein Information
beta-2-glycoprotein 1; B2GPI; apo-H; beta(2)GPI; APC inhibitor; anticardiolipin cofactor; activated protein C-binding protein
UniProt Protein Name
Beta-2-glycoprotein 1
UniProt Synonym Protein Names
APC inhibitor; Activated protein C-binding protein; Anticardiolipin cofactor; Apolipoprotein H; Apo-H; Beta-2-glycoprotein I
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
B2G1; Apo-H; B2GPI; Beta(2)GPI  [Similar Products]
UniProt Entry Name
APOH_HUMAN
NCBI Summary for APOH
Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections. [provided by RefSeq, Jul 2008]
UniProt Comments for APOH
APOH: Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Protein type: Lipid-binding; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: extracellular matrix; extracellular space; chylomicron; cell surface

Molecular Function: heparin binding; identical protein binding; protein binding; phospholipid binding; glycoprotein binding; lipid binding

Biological Process: negative regulation of myeloid cell apoptosis; negative regulation of angiogenesis; triacylglycerol metabolic process; negative regulation of fibrinolysis; positive regulation of blood coagulation; negative regulation of endothelial cell proliferation; negative regulation of blood coagulation; positive regulation of lipoprotein lipase activity; triacylglycerol transport; blood coagulation, intrinsic pathway; plasminogen activation; regulation of fibrinolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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