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anti-lacZ antibody :: Rabbit Beta d Galactosidase (E. coli) Polyclonal Antibody

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Catalog # MBS315025
Unit / Price
  10 mg  /  $500 +1 FREE 8GB USB
anti-lacZ antibody
Product Name

Beta d Galactosidase (E. coli) (lacZ), Polyclonal Antibody

Also Known As

Rabbit A' Beta d Galactosidase

Product Synonym Names
Rabbit Antibody to Beta-d-Galactosidase (E. coli)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
NC_000913.2 (362455..365529, complement)
3D Structure
ModBase 3D Structure for P00722
Clonality
Polyclonal
Host
Rabbit
Specificity
Beta d Galactosidase (E. coli)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase (E. coli). Recognizes Beta-galactosidase from E. coli. Cross react
Purity/Purification
Delipidation, salt fractionation and ion-exchange chromatography followed by extensive dialysis against the buffer.
Form/Format
Purified, Lyophilized
Reconstitute with 1ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature.
Concentration
10mg/ml (OD280nm) (prior to lyophilization) (lot specific)
Immunogen
Beta-Galactosidase (E. coli)
Buffer
Lyophilized from 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
Preservative
0.01% (w/v) Sodium azide
Lyophilized
Yes
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Warnings
This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Preparation and Storage
Store lyophilized product at 2 to 8 degree C. After reconstitution, store for several weeks at 2 to 8 degree C as an undiluted liquid. Prepare working dilution only prior to immediate use. Long-term, aliquot and store at -20 degree C or below.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-lacZ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-lacZ antibody
EIA/ELISA, IEP, Immunohistochemistry - Formalin/Paraffin
NCBI/Uniprot data below describe general gene information for lacZ. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
116,483 Da[Similar Products]
NCBI Official Full Name
beta-D-galactosidase
NCBI Official Symbol
NCBI Official Synonym Symbols
ECK0341; JW0335
  [Similar Products]
NCBI Protein Information
beta-D-galactosidase
UniProt Protein Name
Beta-galactosidase
UniProt Synonym Protein Names
Lactase
Protein Family
UniProt Gene Name
UniProt Entry Name
BGAL_ECOLI
NCBI Summary for lacZ
Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527].
UniProt Comments for lacZ
Catalytic activity: Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687

Cofactor: Binds 2 magnesium ions per monomer. Can also use manganese. Ref.9 Ref.13 Ref.20Binds 1 sodium ion per monomer. Ref.9 Ref.13 Ref.20

Enzyme regulation: Inhibited by phenylethyl thio-beta-D-galactoside (PETG), isopropyl thio-beta-D-galactoside (IPTG), L-ribose, D-galactonolactone, lactose and 2-amino-D-galactose. Ref.24

Subunit structure: Homotetramer. Ref.21

Induction: By allolactose. Ref.8 Ref.24

Sequence similarities: Belongs to the glycosyl hydrolase 2 family.

Biophysicochemical propertiesKinetic parameters:The values for the enzymatic assays using manganese as cofactor are very close.KM=0.04 mM for p-nitrophenyl beta-D-galactoside Ref.9 Ref.14 Ref.15 Ref.17 Ref.18 Ref.24KM=0.12 mM for o-nitrophenyl beta-D-galactosideKM=0.15 mM for 2,3-dinitrophenyl beta-D-galactopyranosideKM=0.41 mM for 2,5-dinitrophenyl beta-D-galactopyranosideKM=11.6 mM for p-nitrophenol-alpha-L-arabinopyranosideKM=16.9 mM for p-nitrophenol-beta-D-fucopyranosideKM=34 µM for p-nitrophenyl beta-D-galactoside (with magnesium as cofactor and 30 degrees Celsius)KM=140 µM for o-nitrophenyl beta-D-galactoside (with magnesium as cofactor and 30 degrees Celsius)KM=940 µM for allolactose (with magnesium as cofactor and 30 degrees Celsius)KM=1350 µM for lactose (with magnesium as cofactor and 30 degrees Celsius)Vmax=30.9 µmol/min/mg enzyme with lactose as substrate (with magnesium as cofactor and 30 degrees Celsius)Vmax=49.7 µmol/min/mg enzyme with allolactose as substrate (with magnesium as cofactor and 30 degrees Celsius)Vmax=59.7 µmol/min/mg enzyme with p-nitrophenyl beta-D-galactoside as substrate (with magnesium as cofactor and 30 degrees Celsius)Vmax=360 µmol/min/mg enzyme with o-nitrophenyl beta-D-galactoside as substrate (with magnesium as cofactor and 30 degrees Celsius)
Product References and Citations for anti-lacZ antibody
• Davidson, B.L., et al., (2000), "Recombinant adeno-associated virus type 2, 4 and 5 vectors: Transduction of variant cell types and regions in the mammalian central nervous system", PNAS, 97(7): 3428-3432

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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