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anti-CA2 antibody :: Sheep CARBONIC ANHYDRASE II Polyclonal Antibody

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Catalog # MBS221550
Unit / Price
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  1 mL  /  $375 +1 FREE 8GB USB
anti-CA2 antibody
Product Name

CARBONIC ANHYDRASE II (CA2), Polyclonal Antibody

Full Product Name

SHEEP ANTI HUMAN CARBONIC ANHYDRASE II

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
259730
3D Structure
ModBase 3D Structure for P00918
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Specificity
Product identity is confirmed by double diffusion vs human CAII. No reactivity is seen in immunodiffusion against CAI.
Form/Format
Purified
Purified IgG - liquid
Perservative Stabilisers
0.09% Sodium Azide
0.1% EACA
1mM EDTA
0.01% Benzamidine
Immunogen
Purified human carbonic anhydrase II (CAII) prepared from erythrocytes.
Buffer Solution
Antiserum Preparation
Antisera to human carbonic anhydrase II were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CA2 antibody
Product identity is confirmed by double diffusion vs human CAII. No reactivity is seen in immunodiffusion against CAI.
Applications Tested/Suitable for anti-CA2 antibody
Immunodiffusion (ID), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-CA2 antibody
Western Blot: Sheep anti human carbonic anyhdrase II recognises a band of ~18kDa in rat adrenomedullary chromaffin cell lysates, see Salman et al. for details. Loading control Rabbit anti Human beta-actin.
Western Blotting: Maximum Dilution: 1/1000
NCBI/Uniprot data below describe general gene information for CA2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,246 Da[Similar Products]
NCBI Official Full Name
carbonic anhydrase 2 isoform 1
NCBI Official Synonym Full Names
carbonic anhydrase II
NCBI Official Symbol
NCBI Official Synonym Symbols
CAC; CAII; Car2; CA-II; HEL-76
  [Similar Products]
NCBI Protein Information
carbonic anhydrase 2; carbonate dehydratase II; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; epididymis luminal protein 76
UniProt Protein Name
Carbonic anhydrase 2
UniProt Synonym Protein Names
Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CAC; CA-II  [Similar Products]
UniProt Entry Name
CAH2_HUMAN
NCBI Summary for CA2
The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
UniProt Comments for CA2
CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family.

Protein type: Energy Metabolism - nitrogen; EC 4.2.1.1; Lyase

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: extracellular space; microvillus; axon; apical part of cell; basolateral plasma membrane; cytoplasm; plasma membrane; cytosol; myelin sheath

Molecular Function: protein binding; carbonate dehydratase activity; zinc ion binding

Biological Process: secretion; positive regulation of osteoclast differentiation; positive regulation of cellular pH reduction; positive regulation of synaptic transmission, GABAergic; one-carbon compound metabolic process; odontogenesis of dentine-containing teeth; response to estrogen stimulus; bicarbonate transport; response to zinc ion; regulation of intracellular pH; morphogenesis of an epithelium; positive regulation of bone resorption; kidney development; response to pH

Disease: Osteopetrosis, Autosomal Recessive 3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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