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anti-CD195 antibody :: Rabbit CD195 Polyclonal Antibody

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Catalog # MBS221619
Unit / Price
  0.1 mg  /  $400 +1 FREE 8GB USB
Product Name

CD195, Polyclonal Antibody

Also Known As

RABBIT ANTI HUMAN CD195 (CCR5)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
352
OMIM
601373
3D Structure
ModBase 3D Structure for P51681
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5 mg/ml (lot specific)
Perservative Stabilisers
0.02% Sodium Azide
Histology Positive Control Tissue
Human lymph node.
Buffer Solution
Antiserum Preparation
Antisera to CCR5 were raised by repeated immunisation of rabbit with highly purified antigen.
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CD195 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD195 antibody
Rabbit anti Human CD195 antibody recognizes the N-terminal region of the chemokine receptor CCR5, also known as CD195. CCR5 is a receptor for the beta chemokines RANTES, MIP-1alpha and MIP-1beta.
Applications Tested/Suitable for anti-CD195 antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-CD195 antibody
Immunohistology - Paraffin: Maximum Dilution: 20 ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Maximum Dilution: 1/1000

Testing Data #1 of anti-CD195 antibody
Staining of human lymph node tissue with Rabbit anti Human CCR5 (NT) at 20 ug/ml. Formalin fixed paraffin processed tissue. (MBS221637)
anti-CD195 antibody Testing Data #1 image
Testing Data #2 of anti-CD195 antibody
Western Blot analysis of THP-1 whole cell lysate probed with Rabbit anti Human CD195 (MBS221619)
anti-CD195 antibody Testing Data #2 image
NCBI/Uniprot data below describe general gene information for CD195. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,524 Da
NCBI Official Full Name
C-C chemokine receptor type 5
NCBI Official Synonym Full Names
chemokine (C-C motif) receptor 5 (gene/pseudogene)
NCBI Official Symbol
NCBI Official Synonym Symbols
CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
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NCBI Protein Information
C-C chemokine receptor type 5; C-C motif chemokine receptor 5 A159A; HIV-1 fusion coreceptor; chemokine receptor CCR5; chemr13
UniProt Protein Name
C-C chemokine receptor type 5
UniProt Synonym Protein Names
CHEMR13; HIV-1 fusion coreceptor; CD_antigen: CD195
UniProt Gene Name
UniProt Synonym Gene Names
CMKBR5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5  [Similar Products]
UniProt Entry Name
CCR5_HUMAN
NCBI Summary for CD195
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for CD195
CCR5: a 7-transmembrane G-linked receptor for a number of inflammatory C-C type chemokines including MIP-1-alpha, MIP-1-beta and RANTES. Transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (along with CD4) for HIV-1 R5 isolates. Interacts with PRAF2. Interacts with HIV-1 surface protein gp120. Efficient ligand binding to CCL3/MIP-1alpha and CCR4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications. Glycosylation on S6 is required for efficient binding of CCL4. Interacts with ADRBK1. Interacts with ARRB1 and ARRB2. Variations in CCR5 are associated with resistance or susceptibility to immunodeficiency virus type 1 (resistance or susceptibility to HIV-1). Variations in CCR5 gene also influence the rate of progression to AIDS after infection. R60S variant, a naturally occurring mutation in a conserved residue in the first intracellular domain of CCR5, results in reduced amounts of the protein in the membrane and consequently may be associated with reduced susceptibility to infection by microbes that depend on these molecules as their receptors. Variations in CCR5 are associated with susceptibility to West Nile virus (WNV) infection

Protein type: Receptor, cytokine; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; Motility/polarity/chemotaxis; GPCR, family 1

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cell surface; integral to plasma membrane; cytoplasm; plasma membrane; endosome; external side of plasma membrane

Molecular Function: protein binding; C-C chemokine receptor activity; chemokine receptor activity; C-C chemokine binding; coreceptor activity; actin binding; phosphoinositide phospholipase C activity

Biological Process: viral reproduction; calcium-mediated signaling; MAPKKK cascade; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; chemotaxis; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; calcium ion transport; dendritic cell chemotaxis; cellular defense response; immune response; entry into host cell; inflammatory response

Disease: West Nile Virus, Susceptibility To; Diabetes Mellitus, Insulin-dependent, 22; Hepatitis C Virus, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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