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anti-CD98 antibody :: Rabbit CD98 LIGHT CHAIN Polyclonal Antibody

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Catalog # MBS221428
Unit / Price
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  0.05 mg  /  $550 +1 FREE 8GB USB
Testing Data #1
Product Name

CD98 LIGHT CHAIN, Polyclonal Antibody

Full Product Name

RABBIT ANTI HUMAN CD98 LIGHT CHAIN (N-TERMINAL)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
600182
3D Structure
ModBase 3D Structure for Q01650
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Specificity
This item detects an epitope within the N-terminal (NT) region of the CD98 light chain (CD98LC), also known as the 4F2 light chain, or LAT-1. CD98LC is a 40kD multi-pass membrane protein which forms a heterodimer with the CD98 heavy chain to allow transport of large branched chain, neutral amino acids into the cell. This transport system leads to the uptake of seven amino acids, including isoleucine, phenylalanine, and methionine.

The expression levels of CD98LC are upregulated in a wide spectrum of primary human cancers. Changes in amino acid transporter expression are thought to be an early change in oncogenesis, as they impart growth and survival advantages.
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Perservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide: N-terminal region of CD98 light chain
Histology Positive Control Tissue
Antiserum Preparation
Antisera to CD98 light chain were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CD98 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD98 antibody
MBS221428 detects an epitope within the N-terminal (NT) region of the CD98 light chain (CD98LC), also known as the 4F2 light chain, or LAT-1. CD98LC is a 40kD multi-pass membrane protein which forms a heterodimer with the CD98 heavy chain to allow transport of large branched chain, neutral amino acids into the cell. This transport system leads to the uptake of seven amino acids, including isoleucine, phenylalanine, and methionine. The expression levels of CD98LC are upregulated in a wide spectrum of primary human cancers. Changes in amino acid transporter expression are thought to be an early change in oncogenesis, as they impart growth and survival advantages.
Applications Tested/Suitable for anti-CD98 antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-CD98 antibody
Western Blot: This item detects a band of approximately 50-55kDa in human liver cell lysates.
Immunohistology - Paraffin: Minimum Dilution: 15; Maximum Dilution: 25ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 1; Maximum Dilution: 10ug/ml

Testing Data #1 of anti-CD98 antibody
Staining of formalin fixed, paraffin embedded human placenta with Rabbit anti Human CD95 light chain (MBS221428)
anti-CD98 antibody Testing Data #1 image
NCBI/Uniprot data below describe general gene information for CD98. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55,010 Da
NCBI Official Full Name
large neutral amino acids transporter small subunit 1
NCBI Official Synonym Full Names
solute carrier family 7 (amino acid transporter light chain, L system), member 5
NCBI Official Symbol
SLC7A5  [Similar Products]
NCBI Official Synonym Symbols
E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E
  [Similar Products]
NCBI Protein Information
large neutral amino acids transporter small subunit 1; 4F2 LC; 4F2 light chain; CD98 light chain; L-type amino acid transporter 1; integral membrane protein E16; large neutral amino acids transporter 1; sodium-independent neutral amino acid transporter LAT1; solute carrier family 7 (cationic amino acid transporter, y+ system), member 5; solute carrier family 7 member 5; y+ system cationic amino acid transporter
UniProt Protein Name
Large neutral amino acids transporter small subunit 1
UniProt Synonym Protein Names
4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter
UniProt Gene Name
SLC7A5  [Similar Products]
UniProt Synonym Gene Names
CD98LC; LAT1; MPE16; 4F2 LC; 4F2LC; hLAT1  [Similar Products]
UniProt Entry Name
LAT1_HUMAN
UniProt Comments for CD98
SLC7A5: Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood- brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L- leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Expression induced in quiescent peripheral blood lymphocytes after treatment with phorbol myristate acetate (PMA) and phytohemagglutinin (PHA). Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore. Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; apical plasma membrane; nucleolus; integral to membrane; plasma membrane; nucleus; cytosol

Molecular Function: L-amino acid transmembrane transporter activity; peptide antigen binding; neutral amino acid transmembrane transporter activity; amino acid transmembrane transporter activity

Biological Process: neutral amino acid transport; amino acid metabolic process; nervous system development; transport; amino acid transport; ion transport; cell differentiation; blood coagulation; leukocyte migration; transmembrane transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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