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anti-C3 antibody :: Rabbit Complement Component 3 (C3) Polyclonal Antibody

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Catalog # MBS2005172 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $330 +1 FREE 8GB USB
  1 mg  /  $785 +1 FREE 8GB USB
Product Name

Complement Component 3 (C3), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Antibody to Complement Component 3 (C3)

Product Gene Name
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Unconjugated Antibody: Complement Component 3 (C3) (MBS2005172)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: Complement Component 3 (C3) (MBS2005172)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2005172 Technical Datasheet
3D Structure
ModBase 3D Structure for P01026
Clonality
Polyclonal
Host
Rabbit
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.63 mg/ml (lot specific)
Organism Species
Rattus norvegicus (Rat)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant Complement Component 3 (Asp966~Trp1313 (Accession # P01026)) expressed in E.coli
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2048851
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of anti-C3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-C3 antibody
WB; IHC; ICC; IP.
Application Notes for anti-C3 antibody
Western blotting: 0.5-2 ug/mL;1:300-1200
Immunohistochemistry: 5-20 ug/mL;1:30-120
Immunocytochemistry: 5-20 ug/mL;1:30-120

Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-C3 antibody
Figure. Western Blot; Sample: Recombinant C3 Rat
anti-C3 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-C3 antibody
(Figure.DAB staining on IHC-P. Samples: Rat Tissue)
anti-C3 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for C3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
complement C3
NCBI Official Synonym Full Names
complement C3
NCBI Official Symbol
NCBI Protein Information
complement C3
UniProt Protein Name
Complement C3
UniProt Synonym Protein Names
Complement C3 beta chainC3-beta-c; C3bc
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
C3bc; ENCF-2; ENCF-1; ASP  [Similar Products]
NCBI Summary for C3
putative complement component C3; likely involved in innate immune response [RGD, Feb 2006]
UniProt Comments for C3
C3: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Defects in C3 are the cause of complement component 3 deficiency (C3D). A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis. Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin- containing structure known as Bruch membrane. Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q12

Cellular Component: extracellular space; protein complex

Molecular Function: C5L2 anaphylatoxin chemotactic receptor binding; cofactor binding; endopeptidase inhibitor activity; lipid binding; protein binding

Biological Process: blood coagulation; chemotaxis; complement activation; complement activation, alternative pathway; complement activation, classical pathway; fatty acid metabolic process; inflammatory response; phagocytosis, engulfment; positive regulation of activation of membrane attack complex; positive regulation of angiogenesis; positive regulation of developmental growth; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of phagocytosis; positive regulation of protein phosphorylation; positive regulation of type IIa hypersensitivity; response to estradiol; response to estrogen; response to glucocorticoid stimulus; response to magnesium ion; response to progesterone; tolerance induction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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