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anti-CDKN2A antibody :: Rabbit Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) Polyclonal Antibody

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Catalog # MBS2015352 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $170 +1 FREE 8GB USB
  0.1 mg  /  $210 +1 FREE 8GB USB
  0.2 mg  /  $315 +1 FREE 8GB USB
  1 mg  /  $745 +1 FREE 8GB USB
Product Name

Cyclin Dependent Kinase Inhibitor 2A (CDKN2A), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2015352 Technical Datasheet
3D Structure
ModBase 3D Structure for P51480
Clonality
Polyclonal
Host
Rabbit
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.72 mg/ml (lot specific)
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Immunogen
Recombinant CDKN2A (Ser3~Thr154) expressed in E.coli
Traits
Liquid
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-CDKN2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CDKN2A antibody
Western blotting (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP)
Application Notes for anti-CDKN2A antibody
Western blotting: 0.5-2 ug/mL;1:360-1400
Immunohistochemistry: 5-20 ug/mL;1:36-140
Immunocytochemistry: 5-20 ug/mL;1:36-140

Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-CDKN2A antibody
Western Blot: Sample: Recombinant CDKN2A, Mouse
anti-CDKN2A antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CDKN2A antibody
DAB staining on fromalin fixed paraffin-embedded Spleen tissue)
anti-CDKN2A antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CDKN2A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
cyclin-dependent kinase inhibitor 2A p16INK4a
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 2A
NCBI Official Symbol
Cdkn2a  [Similar Products]
NCBI Official Synonym Symbols
Arf; p16; MTS1; Pctr1; p19ARF; p16INK4a; p19<ARF>; ARF-INK4a; INK4a-ARF; Ink4a/Arf; p16(INK4a)
  [Similar Products]
NCBI Protein Information
cyclin-dependent kinase inhibitor 2A
UniProt Protein Name
Cyclin-dependent kinase inhibitor 2A
UniProt Synonym Protein Names
Cyclin-dependent kinase 4 inhibitor A; CDK4I; p16-INK4a; p16-INK4
UniProt Gene Name
Cdkn2a  [Similar Products]
UniProt Synonym Gene Names
CDK4I; p16-INK4  [Similar Products]
UniProt Comments for CDKN2A
p16-INK4A: a cell-cycle regulatory protein that interacts with CDK4 and CDK6, inhibiting their ability to interact with cyclins D. Inhibits the phosphorylation of the retinoblastoma protein by CDK4 or CDK6, and entry into the S phase of the cell cycle. The p16INK4A and p14ARF proteins are encoded by CDKN2A, a known tumour suppressor gene in multiple cancers. CDKN2A is inactivated in 72% of cases of lung squamous cell carcinoma: 21% by epigenetic silencing by methylation, 18% inactivating mutation, 4% by exon 1b skipping, and 29% by homozygous deletion. Defects in CDKN2A are the cause of familial atypical multiple mole melanoma-pancreatic carcinoma syndrome, Li-Fraumeni syndrome, and the melanoma-astrocytoma syndrome. The melanoma-astrocytoma syndrome is characterized by a dual predisposition to melanoma and neural system tumors, commonly astrocytoma. Four alternatively spliced p16 isoforms have been reported. Two alternatively spliced isoforms of ARF have been reported.

Protein type: Cell cycle regulation; Nucleolus; Tumor suppressor

Chromosomal Location of Human Ortholog: 4 C4|4 42.15 cM

Cellular Component: cytoplasm; granular component; mitochondrion; nuclear body; nucleolus; nucleoplasm; nucleus; protein complex; senescence-associated heterochromatin focus

Molecular Function: cyclin-dependent protein serine/threonine kinase inhibitor activity; DNA binding; DNA binding transcription factor activity; NF-kappaB binding; p53 binding; protein binding; protein kinase binding; protein N-terminus binding; RNA binding; SUMO transferase activity; transcription factor binding; ubiquitin ligase inhibitor activity; ubiquitin-protein transferase inhibitor activity

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; aging; amyloid fibril formation; apoptosis; apoptotic mitochondrial changes; cell aging; cell cycle; cell cycle arrest; cellular response to hydrogen peroxide; cellular senescence; epidermis development; G1/S transition of mitotic cell cycle; glucose homeostasis; mitochondrial depolarization; mitochondrion degradation; negative regulation of B cell proliferation; negative regulation of cell cycle; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of cell-matrix adhesion; negative regulation of cyclin-dependent protein serine/threonine kinase activity; negative regulation of hepatocyte proliferation; negative regulation of immature T cell proliferation in the thymus; negative regulation of mammary gland epithelial cell proliferation; negative regulation of NF-kappaB transcription factor activity; negative regulation of phosphorylation; negative regulation of protein binding; negative regulation of protein kinase activity; negative regulation of protein neddylation; negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process; negative regulation of transcription, DNA-dependent; negative regulation of ubiquitin protein ligase activity; negative regulation of ubiquitin-protein ligase activity; positive regulation of apoptosis; positive regulation of apoptotic process involved in mammary gland involution; positive regulation of cell cycle arrest; positive regulation of cellular senescence; positive regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of gene expression; positive regulation of macrophage apoptotic process; positive regulation of protein localization to nucleus; positive regulation of protein sumoylation; positive regulation of smooth muscle cell apoptotic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; protein destabilization; protein polyubiquitination; protein stabilization; regulation of cyclin-dependent protein kinase activity; regulation of G2/M transition of mitotic cell cycle; regulation of gene expression; regulation of nucleocytoplasmic transport; regulation of protein export from nucleus; regulation of protein stability; regulation of transcription, DNA-templated; replicative senescence; response to drug; response to organic cyclic compound; response to organonitrogen compound; rRNA processing; rRNA transcription; senescence-associated heterochromatin focus assembly; somatic stem cell division; somatic stem cell maintenance; transcription, DNA-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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