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anti-SERPIND1 antibody :: Goat Heparin Cofactor II Polyclonal Antibody

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Catalog # MBS512096
Unit / Price
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  0.1 mg  /  $345 +1 FREE 8GB USB
anti-SERPIND1 antibody
Product Name

Heparin Cofactor II (SERPIND1), Polyclonal Antibody

Full Product Name

Goat anti-human Heparin Cofactor II, Peroxidase Conjugated Affinity-Purified IgG

Product Synonym Names
Heparin Cofactor II, human
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 22; NC_000022.10 (21128383..21142008). Location: 22q11.2; 22q11.21
OMIM
142360
3D Structure
ModBase 3D Structure for P05546
Clonality
Polyclonal
Host
Goat
Specificity
This antibody is specific for HCII as demonstrated by immunoelectrophoresis and ELISA.
Purity/Purification
Affinity purified, peroxidase conj. IgG.
Vial containing ml of IgG purified by affinity chromatography on immobilized HCII. Total protein is 0.5 mg.
Form/Format
Affinity-purified IgG (APIgG), clear liquid.
Concentration
APIgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4. (lot specific)
Immunogen
Human Heparin Cofactor II purified from plasma.
Buffer
10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.
Neutralizing Activity
Not determined
Preparation and Storage
Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.
Other Notes
Small volumes of anti-SERPIND1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SERPIND1 antibody
Heparin Cofactor II (HCII), also known as heparin cofactor A and dermatan sulphate cofactor, is a single chain glycoprotein produced in the liver and circulates in plasma at concentration of 50-100 ug/ml (0.8-1.5 uM). A member of the SERPIN family of proteinase inhibitors, HCII exhibits a relatively narrow specificity and is only known to inhibit the serine proteinases thrombin and chymotrypsin. The inhibitory activity toward thrombin is accelerated up to 1000-fold in the presence of optimal concentrations of heparin. Unlike Antithrombin, thrombin inhibition by HCII is also enhanced by dermatan sulphate. HCII has an apparent molecular weight of 65,600 daltons and interaction with thrombin results in a covalent 1:1 enzyme-inhibitor complex of 102,600 daltons 1-3.
Application Notes for anti-SERPIND1 antibody
Suitable as a source of enriched antibodies to human HCII.
NCBI/Uniprot data below describe general gene information for SERPIND1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
NCBI Official Full Name
heparin cofactor 2
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade D (heparin cofactor), member 1
NCBI Official Symbol
SERPIND1  [Similar Products]
NCBI Official Synonym Symbols
HC2; LS2; HCF2; HCII; HLS2; D22S673
  [Similar Products]
NCBI Protein Information
heparin cofactor 2; serpin D1; leuserpin 2; OTTHUMP00000198450; OTTHUMP00000198451; protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1
UniProt Protein Name
Heparin cofactor 2
UniProt Synonym Protein Names
Heparin cofactor II; HC-II; Protease inhibitor leuserpin-2; HLS2; Serpin D1
Protein Family
UniProt Gene Name
SERPIND1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
HEP2_HUMAN
NCBI Summary for SERPIND1
The product encoded by this gene is a serine proteinase inhibitor which rapidly inhibits thrombin in the presence of dermatan sulfate or heparin. The gene contains five exons and four introns. This protein shares homology with antithrombin III and other members of the alpha 1-antitrypsin superfamily. Mutations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq]
UniProt Comments for SERPIND1
Function: Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Ref.11Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils. Ref.11

Tissue specificity: Expressed predominantly in liver. Also present in plasma. Ref.15

Domain: The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition. Ref.11

Post-translational modification: Phosphorylation sites are present in the extracelllular medium.

Involvement in disease: Defects in SERPIND1 are the cause of heparin cofactor 2 deficiency (HCF2D) [

MIM:612356]. HCF2D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. Ref.16 Ref.17 Ref.19 Ref.20

Sequence similarities: Belongs to the serpin family.

Sequence caution: The sequence CAG30459.1 differs from that shown. Reason: Erroneous initiation.
Product References and Citations for anti-SERPIND1 antibody
1. Tollefsen DM, Majerus DW, Blank MK; Heparin Cofactor II; JBC 257:5, p2162-2169, 1982.
2. Pratt CW, Whinna HC, Mead JB, Treanor RE, Church FC: Physicochemical aspects of Heparin Cofactor II; Ann N.Y. Acad. Sci.: 556, p104-115, 1989.
3. Griffith MJ, Noyes CM, Church FC; Reactive Site Peptide Structural Similarity between Heparin Cofactor II and Antithrombin III; JBC:260, pp 2218-2225, 1985.

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Disclaimer
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