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anti-M-CSF antibody :: Goat M-CSF Polyclonal Antibody

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Catalog # MBS224164
Unit / Price
  0.1 mg  /  $555 +1 FREE 8GB USB
anti-M-CSF antibody
Product Name

M-CSF, Polyclonal Antibody

Also Known As

GOAT ANTI MOUSE M-CSF

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
257
3D Structure
ModBase 3D Structure for P07141
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Form/Format
Purified
Purified IgG - lyophilised
Concentration
IgG concentration 1.0mg/ml after reconstitution (lot specific)
Reconstitution
Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Immunogen
Recombinant mouse M-CSF
Buffer Solution
Antiserum Preparation
Antiserum to mouse M-CSF was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species
Mouse
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Other Notes
Small volumes of anti-M-CSF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-M-CSF antibody
Goat anti Mouse M-CSF antibody recognizes mouse M-CSF (Macrophage colony-stimulating factor), a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells. Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor). A genetic defect in M-CSF is responsible for osteopetrosis in mice (op/op), which are severely deficient in mature macrophages and have a restricted capacity for bone remodelling.
Applications Tested/Suitable for anti-M-CSF antibody
ELISA (EIA), Functional Assays (FN), Western Blot (WB)
Application Notes for anti-M-CSF antibody
ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;
Functional Assays: Minimum Dilution: 0.05; Maximum Dilution: 0.08ug/ml;
Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
NCBI/Uniprot data below describe general gene information for M-CSF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,353 Da
NCBI Official Full Name
macrophage colony-stimulating factor 1 isoform 2
NCBI Official Synonym Full Names
colony stimulating factor 1 (macrophage)
NCBI Official Symbol
NCBI Official Synonym Symbols
op; Csfm; MCSF; C87615
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NCBI Protein Information
macrophage colony-stimulating factor 1; osteopetrosis
UniProt Protein Name
Macrophage colony-stimulating factor 1
UniProt Gene Name
UniProt Synonym Gene Names
Csfm; CSF-1; MCSF  [Similar Products]
UniProt Entry Name
CSF1_MOUSE
UniProt Comments for M-CSF
M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine

Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; plasma membrane; extracellular region; integral to membrane; receptor complex

Molecular Function: protein binding; protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of monocyte differentiation; ossification; macrophage differentiation; immune system process; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; reproductive developmental process; positive regulation of odontogenesis of dentine-containing teeth; positive regulation of multicellular organism growth; osteoclast differentiation; odontogenesis; cell proliferation; positive regulation of mononuclear cell proliferation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; positive regulation of cell proliferation; innate immune response; positive regulation of Ras protein signal transduction; regulation of ossification; positive regulation of cell-matrix adhesion; inflammatory response; positive regulation of macrophage differentiation; positive regulation of cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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