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anti-SCNN1A antibody :: Rabbit Na+ Channel Polyclonal Antibody

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Catalog # MBS610104
Unit / Price
  0.2 mg  /  $705 +1 FREE 8GB USB
Product Name

Na+ Channel (SCNN1A), Polyclonal Antibody

Popular Item
Also Known As

Na+ Channel (III-IV loop)

Product Synonym Names
Anti -Na+ Channel (III-IV loop)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 12; NC_000012.11 (6456009..6486523, complement). Location: 12p13
OMIM
264350
3D Structure
ModBase 3D Structure for P37088
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Specificity
Recognizes the vertebrate sodium channel intracellular III-IV loop at ~ 220kDa. Species crossreactivity: Expected for all vertebrates
Purity/Purification
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.05% sodium azide, 30% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
KLH conjugated synthetic peptide 'EOIII' (TEEQKKYYNAMKKLGSKKC) corresponding to a highly conserved segment in the intracellular III-IV loop, which to date, is identical in all known vertebrates.
Positive Control
Rat brain Microsomal Lysate.
Preparation and Storage
May be stored at 4 degree C for short term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-SCNN1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SCNN1A antibody
The Na + channel protein mediates the voltage-dependent sodium ion permeability of excitable membranes by forming a sodium-selective channel through which Na + ions may pass in accordance with their electrochemical gradient. The Na + channel protein contains four internal repeats, each with five hydrophobic segments (S1, S2, S3, S5, S6) and one positively charged segment (S4). The S4 segments are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
Applications Tested/Suitable for anti-SCNN1A antibody
Western Blot (WB), Immunoprecipitation (IP), Imunohistochemistry (IHC)
Application Notes for anti-SCNN1A antibody
Western Blot: 0.5-2ug/ml detected Sodium Channel (III-IV) Linker Region) in Rat brain microsomal tissue extract. 40ug rat brain microsomal tissue extract was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS610104 (2ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Note: Do not boil tissue extracts, but instead heat to 37 degree C for 15-30 minutes prior to loading the gel.
immunoprecipitation: 1-4ug immunoprecipitated Sodium Channel (III-IV Linker Region) from 400ug of Rat Brain Microsomal Tissue extract.
Optimal dilutions to be determined by the researcher.

Mouse sciatic nerves stained with MBS610104 at 20ug/ml.
anti-SCNN1A antibody Test Data image
Western Blot of anti-SCNN1A antibody
WB analysis of 20ug rat brain microsomal preparation using 0.5-2ug/ml of MBS610104.
anti-SCNN1A antibody Western Blot (WB) image
NCBI/Uniprot data below describe general gene information for SCNN1A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
75,704 Da[Similar Products]
NCBI Official Full Name
Na+ channel
NCBI Official Synonym Full Names
sodium channel, nonvoltage-gated 1 alpha
NCBI Official Symbol
SCNN1A  [Similar Products]
NCBI Official Synonym Symbols
BESC2; ENaCa; SCNEA; SCNN1; FLJ21883; ENaCalpha
  [Similar Products]
NCBI Protein Information
amiloride-sensitive sodium channel subunit alpha; alpha-ENaC; alpha-NaCH; alpha ENaC-2; OTTHUMP00000238446; OTTHUMP00000238447; OTTHUMP00000238448; OTTHUMP00000238449; OTTHUMP00000238451; epithelial Na(+) channel subunit alpha; nasal epithelial sodium channel alpha subunit; nonvoltage-gated sodium channel 1 subunit alpha; amiloride-sensitive sodium channel subunit alpha 2; amiloride-sensitive epithelial sodium channel alpha subunit
UniProt Protein Name
Amiloride-sensitive sodium channel subunit alpha
UniProt Synonym Protein Names
Alpha-NaCH; Epithelial Na(+) channel subunit alpha; Alpha-ENaC; ENaCA; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA
UniProt Gene Name
SCNN1A  [Similar Products]
UniProt Synonym Gene Names
SCNN1  [Similar Products]
UniProt Entry Name
SCNNA_HUMAN
NCBI Summary for SCNN1A
Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]
UniProt Comments for SCNN1A
ENaC-alpha: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. Probable heterotrimer containing one alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. By aldosterone. Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart. Activated by WNK1, WNK2, WNK3 and WNK4. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1A subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, ion channel; Channel, sodium; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cortical actin cytoskeleton; integral to plasma membrane; apical plasma membrane; plasma membrane; cytosol; external side of plasma membrane

Molecular Function: amiloride-sensitive sodium channel activity; protein binding; WW domain binding; actin binding

Biological Process: sensory perception of taste; sodium ion transport; response to stimulus; sodium ion homeostasis; multicellular organismal water homeostasis; excretion; transmembrane transport

Disease: Bronchiectasis With Or Without Elevated Sweat Chloride 2; Pseudohypoaldosteronism, Type I, Autosomal Recessive
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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