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anti-NOS3 antibody :: Rabbit Nitric Oxide Synthase, Endothelial (eNOS) (a.a. 2-11) Polyclonal Antibody

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Catalog # MBS316207
Unit / Price
  0.1 mL  /  $430 +1 FREE 8GB USB
anti-NOS3 antibody
Product Name

Nitric Oxide Synthase, Endothelial (eNOS) (a.a. 2-11) (NOS3), Polyclonal Antibody

Full Product Name

Rabbit anti eNOS

Product Synonym Names
Rabbit Antibody to Human Endothelial Nitric Oxide Synthase (eNOS)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 7; NC_000007.13 (150688144..150711687). Location: 7q36
OMIM
163729
3D Structure
ModBase 3D Structure for P29474
Clonality
Polyclonal
Host
Rabbit
Specificity
Nitric Oxide Synthase, Endothelial (eNOS) (a.a. 2-11)
Recognizes Human Endothelial Nitric Oxide Synthase (eNOS). Specific for the amino terminal of eNOS. It recognizes the non-myrisylated form best, but does cross-react ~50% with the myristylated form
Form/Format
Neat, Lyophilized.
Reconstitute with 0.1ml PBS containing 10mg/ml BSA or with additional buffer for more dilute antibody.
Immunogen
eNOS (2-11 Cys^12) covalently attached to a carrier protein.
Buffer
Not applicable.
Preservative
No
Lyophilized
Yes
Preparation and Storage
Store lyophilized product at 2 to 8 degree C (preferably in a dessicator). After reconstitution, aliquot and store at -20 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-NOS3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for NOS3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
133,289 Da[Similar Products]
NCBI Official Full Name
Nitric oxide synthase, endothelial
NCBI Official Synonym Full Names
nitric oxide synthase 3 (endothelial cell)
NCBI Official Symbol
NCBI Official Synonym Symbols
eNOS; ECNOS
  [Similar Products]
NCBI Protein Information
nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; OTTHUMP00000212826; OTTHUMP00000213183; OTTHUMP00000213184; OTTHUMP00000213185
UniProt Protein Name
Nitric oxide synthase, endothelial
UniProt Synonym Protein Names
Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III
Protein Family
UniProt Gene Name
UniProt Entry Name
NOS3_HUMAN
NCBI Summary for NOS3
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
UniProt Comments for NOS3
Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Catalytic activity: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.

Cofactor: Heme group.Binds 1 FAD.Binds 1 FMN.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.

Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. Ref.19 Ref.20

Subunit structure: Homodimer. Interacts with NOSIP and NOSTRIN. Ref.19 Ref.20

Subcellular location: Cell membrane. Membrane › caveola. Cytoplasm › cytoskeleton. Golgi apparatus. Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. Ref.19 Ref.20 Ref.21 Ref.22

Tissue specificity: Platelets, placenta, liver and kidney. Ref.18

Polymorphism: Variation in NOS3 seem to be associated with susceptibility to coronary spasm.

Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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