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anti-OPRD1 antibody :: Opioid Receptor Delta IgG fraction Polyclonal Antibody

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Catalog # MBS352198
Unit / Price
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  0.1 mL  /  $700 +1 FREE 8GB USB
anti-OPRD1 antibody
Product Name

Opioid Receptor Delta IgG fraction (OPRD1), Polyclonal Antibody

Full Product Name

Opioid Receptor Delta IgG fraction

Product Synonym Names
Opioid Delta Receptor (360-372); IgG Fraction of Rabbit Anti-Delta Opioid Receptor Serum
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (29138654..29190208). Location: 1p36.1-p34.3
OMIM
165195
3D Structure
ModBase 3D Structure for P41143
Clonality
Polyclonal
Specificity
Polypeptide ::: % Cross Reactivity
Delta Opioid Receptor (360-372) ::: 100
Delta Opioid Receptor ::: 60
Form/Format
Antiserum Specificity
Other Notes
Small volumes of anti-OPRD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-OPRD1 antibody
This IgG fraction was prepared by precipitation. dialysis and chromatography from an antiserum that was raised in a rabbit which was immunized with a peptide analogue of the carboxyl terminal of the delta opioid receptor covalently attached onto a carrier protein. The antiserum has been shown to be specific for the COOH terminal of the delta opioid receptor and has been characterized by western blotting. ELISA. and cell staining techniques. The IgG fraction is suitable for immunocytochemical and western immunoblotting detection of the receptor. Dilute the lyophilized antibody with 0.1 ml of 10 mg/ml BSA in PBS for the equivalent concentration in whole antiserum. or with additional buffer for more dilute antibody. This vial contains 1.69 mg of purified IgG
Applications Tested/Suitable for anti-OPRD1 antibody
Immunocytochemical Staining, Western Immunoblot, Western Blot
Application Notes for anti-OPRD1 antibody
Immunocytochemistry: This IgG fraction has been found to stain specific cells in various regions of PLP fixed rat brain sections known to express the delta opioid receptor using ABC techniques at a concentration of 20 ug per ml..

Western Blot: Western immunoblots using whole rat brain homogenate have been successful at a concentration of 50 ug per ml.

Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the IgG fraction after dilution to at least 50 ugm per ml (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
NCBI/Uniprot data below describe general gene information for OPRD1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
NCBI Official Full Name
delta-type opioid receptor
NCBI Official Synonym Full Names
opioid receptor, delta 1
NCBI Official Symbol
OPRD1  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
delta-type opioid receptor; DOR-1; D-OR-1; OTTHUMP00000003778; delta opioid receptor 1
UniProt Protein Name
Delta-type opioid receptor
UniProt Synonym Protein Names
DOR-1
UniProt Gene Name
OPRD1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
OPRD_HUMAN
UniProt Comments for OPRD1
Function: Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Highly stereoselective. receptor for enkephalins.

Subunit structure: Interacts with GPRASP1. Ref.6 Ref.7

Subcellular location: Cell membrane; Multi-pass membrane protein.

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-OPRD1 antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7 publications with OPRD1 and Brain
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