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anti-OPRM1 antibody :: Opioid Receptor Mu IgG fraction Polyclonal Antibody

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Catalog # MBS352201
Unit / Price
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  0.1 mL  /  $700 +1 FREE 8GB USB
anti-OPRM1 antibody
Product Name

Opioid Receptor Mu IgG fraction (OPRM1), Polyclonal Antibody

Full Product Name

Opioid Receptor Mu IgG fraction

Product Synonym Names
Opioid Mu Receptor (391-398); IgG Fraction of Rabbit Anti-Mu Opioid Receptor Serum
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 6; NC_000006.11 (154331636..154568001). Location: 6q24-q25
OMIM
600018
3D Structure
ModBase 3D Structure for P35372
Clonality
Polyclonal
Specificity
Polypeptide ::: % Cross Reactivity
Mu Opioid Receptor (391-398) ::: 100
Mu Opioid Receptor ::: 50
Form/Format
Antiserum Specificity
Other Notes
Small volumes of anti-OPRM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-OPRM1 antibody
This IgG fraction was prepared by precipitation. dialysis. and chromatography from an antiserum that was raised a rabbit which was immunized with a peptide analogue of the carboxyl terminal of the mu opioid receptor covalently attached onto a carrier protein. This IgG fraction has been shown to be specific for the COOH terminal of the mu opioid receptor. is suitable for immunocytochemical and western immunoblotting detection of the receptor. and has been characterized by western blotting. ELISA. and cell staining techniques. Dilute the lyophilized antiserum with 0.1 ml of 10 mg/ml BSA is PBS for the equivalent of whole antiserum. or with additional buffer for more dilute antiserum. This vial contains 2.14 mg of purified IgG
Applications Tested/Suitable for anti-OPRM1 antibody
Immunocytochemical Staining, Western Immunoblot, Western Blot
Application Notes for anti-OPRM1 antibody
Immunocytochemistry: This IgG fraction has been found to stain specific cells in various regions of PLP fixed rat brain sections known to express the mu opioid receptor using ABC techniques at a concentration of 20 ug per ml.

Western Blot: Western immunoblots using whole rat brain homogenate have been successful at a concentration of 50 ug per ml.

Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the IgG fraction after dilution to at least 50 ugm per ml (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
NCBI/Uniprot data below describe general gene information for OPRM1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
NCBI Official Full Name
mu-type opioid receptor isoform MOR-1
NCBI Official Synonym Full Names
opioid receptor, mu 1
NCBI Official Symbol
OPRM1  [Similar Products]
NCBI Official Synonym Symbols
MOR; LMOR; MOR1; OPRM; KIAA0403
  [Similar Products]
NCBI Protein Information
mu-type opioid receptor; MOP; hMOP; M-OR-1; OTTHUMP00000017463; OTTHUMP00000017464; OTTHUMP00000225129; OTTHUMP00000225131; OTTHUMP00000225132; OTTHUMP00000225133; OTTHUMP00000225135; OTTHUMP00000225136; OTTHUMP00000225139; OTTHUMP00000225164; OTTHUMP00000225165; OTTHUMP00000225166; OTTHUMP00000225167; OTTHUMP00000225168; OTTHUMP00000225169; OTTHUMP00000225170; mu opiate receptor
UniProt Protein Name
Mu-type opioid receptor
UniProt Synonym Protein Names
Mu opiate receptor; Mu opioid receptor
Protein Family
UniProt Gene Name
OPRM1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
OPRM_HUMAN
NCBI Summary for OPRM1
The OPRM1 gene encodes the mu opioid receptor, which is the primary site of action for the most commonly used opioids, including morphine, heroin, fentanyl, and methadone. It is also the primary receptor for endogenous opioid peptides beta-endorphin (see POMC, MIM 176830) and the enkephalins (see PENK, MIM 131330). The OPRM1 receptor is a membrane of the G protein-coupled receptor family (Bond et al., 1998 [PubMed 9689128]). There are at least 3 types of opioid receptors, mu, kappa (OPRK1; MIM 165196), and delta (OPRD1; MIM 165195), each with a distinct pharmacologic profile (Chen et al., 1993 [PubMed 8393525]).[supplied by OMIM]
UniProt Comments for OPRM1
Function: Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin.

Subunit structure: Forms a complex with G(alpha)z/i2 subunits and the RGSZ proteins, RGSZ17 and RGSZ20. The formation of this complex results in mu-opioid receptor desensitization. Interacts with RGSZ17 and RGSZ20

By similarity. The C-terminal tail interacts with the C-terminal section of DNAJB4. Ref.17

Subcellular location: Cell membrane; Multi-pass membrane protein.

Polymorphism: Variant Asp-40 does not show altered binding affinities for most opioid peptides and alkaloids tested, but it binds beta-endorphin, an endogenous opioid that activates the mu opioid receptor, approximately 3 times more tightly than the most common allelic form.

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.

Sequence caution: The sequence CAI20458.1 differs from that shown. Reason: Erroneous initiation. The sequence EAW47705.1 differs from that shown. Reason: Erroneous initiation. Isoform 9: The sequence AAQ77392.1 differs from that shown. Reason: a polymorphism leading to premature termination of translation (position: 411:Q->Stop)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with anti-OPRM1 antibody
Organs/Tissues associated with anti-OPRM1 antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >59 publications with OPRM1 and Brain
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