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Sheep Thrombin Polyclonal Antibody

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Catalog # MBS512156
Unit / Price
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  0.2 mg  /  $380 +1 FREE 8GB USB
anti-Thrombin antibody
Product Name

Thrombin, Polyclonal Antibody

Popular Item
Full Product Name

Sheep anti-bovine Thrombin, Peroxidase Conjugated IgG

Product Synonym Names
Thrombin, bovine
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Polyclonal
Host
Sheep
Specificity
Prior to conjugation, this antibody was specific for bovine thrombin or prothrombin as demonstrated by immunoelectrophoresis and ELISA.
Purity/Purification
Peroxidase conjugated IgG.
Vial containing 1mg/ml of whole IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups. Total protein is 0.2 mg.
Form/Format
IgG-HRP conjugate as a clear, slightly red-brown liquid.
Concentration
IgG-HRP concentration is 2mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 14. (lot specific)
Immunogen
Thrombin prepared from purified bovine prothrombin, active site blocked with PPACK.
Rz Ratio (Reinheitszahl, A403/A280)
0.49 as determined spectrophotometrically.
Buffer
A buffered stabilizer solution containing 50% (v/v) glycerol.
Preparation and Storage
Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use. Avoid exposure to sodium azide as this is an inhibitor of peroxidase activity.
Other Notes
Small volumes of anti-Thrombin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Thrombin antibody
Thrombin (EC3.4.21.5, alpha-thrombin) is the product of proteolytic activation of the zymogen prothrombin. Human thrombin is a two-chain serine protease with a mass of 37 kDa. The active site is located within the heavy chain. Thrombin has a high specificity for certain arginine bonds in protein substrates. The primary substrate is fibrinogen which thrombin converts to fibrin through the cleavage of four arginyl-glycyl peptide bonds. Thrombin is also an important activator of platelets, factor XIII, Protein C and TAFI (Plasma procarboxypeptidase B). In a positive feedback mechanism, thrombin increases the rate of its own production by activation of factors VIII and V. The rate of thrombin production is subsequently limited indirectly through the activation of Protein C by thrombin, which then inactivates the activated cofactors VIII and V. The binding of thrombin to thrombomodulin on the cell surface dramatically alters thrombin's specificity, increasing it's activity toward Protein C and TAFI, and decreasing it's activity toward fibrinogen and activating cofactors VIII and V. In plasma, thrombin activity is inhibited primarily by antithrombin and to a lesser extent heparin cofactor II. The rate of inhibition by both of these inhibitors is profoundly increased in the presence of optimal concentrations of heparin. Other physiological inhibitors of thrombin in the absence of heparin include alpha2-macroglobulin and alpha1-antitrypsin1-4.
Application Notes for anti-Thrombin antibody
Suitable as a source of peroxidase labelled antibodies to bovine thrombin or prothrombin.
NCBI/Uniprot data below describe general gene information for Thrombin. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Official Full Name
thrombin
Protein Family
Product References and Citations for anti-Thrombin antibody
1. Mann KG; Prothrombin and Thrombin; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 184-199, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
2. Stubbs MT, Bode W; A Player of Many Parts: The Spotlight Falls on Thrombin's Structure; Thrombosis Research 69, pp 1-58, 1993.
3. Downing MW, Bloom JW, Mann KG; Comparison of the Inhibition of Thrombin by Three Plasma Protease Inhibitors; Biochemistry 17, pp 2649- 2653, 1978.
4. Weitz JI, Hudoba M, Massel D, Maranganore J, Hirsh J; Clot-bound Thrombin is Protected from Inhibition by Heparin-ATIII but is Susceptible to - ATIII independent Inhibitors; J. Clin. Invest 86, pp 385-391, 1990.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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