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anti-VWF antibody :: Goat von Willebrand Factor Polyclonal Antibody

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Catalog # MBS512178
Unit / Price
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  0.1 mg  /  $340 +1 FREE 8GB USB
anti-VWF antibody
Product Name

von Willebrand Factor (VWF), Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-human von Willebrand Factor, Biotinylated Affinity-Purified IgG

Product Synonym Names
von Willebrand Factor, human
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 27; NC_006609.2 (41865033..42002564)
3D Structure
ModBase 3D Structure for Q28295
Clonality
Polyclonal
Host
Goat
Specificity
Prior to conjugation, this antibody was specific for vWF as demonstrated by immunoelectrophoresis and ELISA.
Purity/Purification
Affinity purified, Biotinylated IgG.
Vial containing mL of affinity-purified IgG conjugated to biotin. Total protein is 0.1 mg.
Form/Format
APIgG-biotin conjugate as a clear, colourless liquid.
Concentration
APIgG-biotin concentration is 1 mg/mL, determined by absorbance using an extinction coefficient (E1%280) of 14. (lot specific)
Immunogen
Human vWF purified from plasma.
Description
Vial containing 0.1mL of affinity-purified IgG conjugated to biotin. Total protein is 0.1 mg
Buffer
Phosphate-buffered saline containing 1 mg/mL bovine albumin and 0.1% sodium azide (w/v), pH 7.4.
Incorporation of Biotin
7.82 moles biotin per mole IgG as determined by HABA assay.
Preparation and Storage
Store at 2 degree C to 8 degree C.
Other Notes
Small volumes of anti-VWF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-VWF antibody
von Willebrand Factor (vWF, also previously referred to as Factor VIII related antigen) is a large adhesive protein produced in endothelial cells and megakaryocytes. There are two critical functions of vWF, the first being its involvement in the process of platelet adhesion and aggregation through interaction with platelet receptor glycoprotein Ib, the second being the binding and stabilization of Factor VIII (antihemophilic factor) for secretion and transport in plasma. The vWF precursor protein is synthesized with a 95,000 dalton propeptide (also known as vWF antigen-II), believed to be involved in the intracellular multimerization of the vWF subunits. The mature vWF multimers are then packed into storage organelles within the cell (Weibel-Palade bodies) after which the propeptide is cleaved and released. vWF circulates as multimers of disulphide linked 220,000 dalton subunits and the molecular weight of these multimers ranges from 0.5- 20 million daltons. The plasma concentration of vWF is typically 10 ug/mL, but increased levels are often observed in pregnancy and other conditions of physiological stress. von Willebrand's disease (vWD) is perhaps the most common inherited bleeding disorder in humans and is the result of either quantitative deficiencies of vWF (vWD Types I & III), or one of a number of qualitative disorders of vWF structure and function (vWD Type II).
Applications Tested/Suitable for anti-VWF antibody
Suitable as a source of biotinylated antibodies to vWF.
Application Notes for anti-VWF antibody
Related Products:
MBS512174
MBS512175
MBS512176
MBS512177
MBS512201
MBS514025
MBS514042
NCBI/Uniprot data below describe general gene information for VWF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
309533 Da[Similar Products]
NCBI Official Full Name
von Willebrand factor
NCBI Official Symbol
NCBI Official Synonym Symbols
F8VWF
  [Similar Products]
NCBI Protein Information
von Willebrand factor
UniProt Protein Name
von Willebrand factor
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
F8VWF  [Similar Products]
UniProt Entry Name
VWF_CANFA
UniProt Comments for VWF
Function: Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex, glycoprotein Ibalpha/IX/V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma

Subunit structure: Multimeric. Interacts with F8. Ref.2

Subcellular location: Secreted. Secreted › extracellular space › extracellular matrix. Note: Localized to storage granules. Ref.2

Tissue specificity: Plasma.

Domain: The propeptide is required for multimerization of vWF and for its targeting to storage granules.

Post-translational modification: All cysteine residues are involved in intrachain or interchain disulfide bonds

By similarity.N- and O-glycosylated

Involvement in disease: Note=Defects in VWF are the cause of von Willebrand disease (VWD) in the Scottish Terrier. VWD is characterized by frequent bleeding. Type I VWD is associated with a deficiency of VWF; type II by normal to decreased plasma level of VWF; type III by a virtual absence of VWF. Ref.4

Sequence similarities: Contains 1 CTCK (C-terminal cystine knot-like) domain.Contains 4 TIL (trypsin inhibitory-like) domains.Contains 3 VWFA domains.Contains 3 VWFC domains.Contains 4 VWFD domains.
Product References and Citations for anti-VWF antibody
1. Montgomery RR, Coller BS, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 134-168, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
2. Sadler JE; von Willebrand Factor (Minireview); JBC 266:34, pp 22777- 22780, 1991.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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