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anti-S6K1 antibody :: Rabbit anti-Arabidopsis Thr449 S6 kinase 1 Polyclonal Antibody

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Catalog # MBS502218
Unit / Price
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  0.1 mL  /  $360 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Thr449 S6 kinase 1 (S6K1), Polyclonal Antibody

Popular Item
Full Product Name

Anti-Phospho-Thr449 S6 kinase 1 (S6K1)

Product Synonym Names
S6 Kinase 1 (Thr449)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P42818
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Arabidopsis
Specificity
Specific for the ~53k S6K1 protein phosphorylated at Thr449. The immunolabeling of the S6K1 band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling.
Purity/Purification
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. (Adequate amount of material to conduct 10 mini Western blots)
Immunogen Species
Arabidopsis
Immunogen Info
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr449 conjugated to KLH
Biological Significance
Ribosomal s6 kinase is a member of a family of protein kinases involved in signal transduction. The subfamily S6K has two known homologues: S6K1 and S6K2. First characterized in mammals, S6K1 is controlled by target-of-rapamycin (TOR) kinase, which plays a central regulatory role in growth signaling pathways (Dufner and Thomas 1999). Osmotic stress inhibition of S6K is mediated by the TOR kinase pathway (Mahfouz et al., 2006). The activation of mammalian S6K1 involves phosphorylation at thr389 (Pearson et al., 2005), however its orthologue in Arabidopsis suggests that plant S6K1 thr449 is its functional equivalent (Schepetilnikov et al., 2011). The phytohormone auxin triggers TOR activation, which is followed by S6K1 phosphorylation at thr449, which in turn is critical for translation reinitiation (Schepetilnikov et al., 2013). Rapamycin effectively inactivates S6K1 thr449 phosphorylation in Arabidopsis seedlings, which suppresses TOR PK activity and ultimately plant growth (Xiong Y and Sheen J, 2011).
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr449 of Arabidopsis S6K1.
Preparation and Storage
For long term storage -20°C is recommended. Stable at -20°C for at least 1 year.
Other Notes
Small volumes of anti-S6K1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-S6K1 antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-S6K1 antibody
Western Blot (WB)
Application Notes for anti-S6K1 antibody
WB: 1:1000

Western Blot (WB) of anti-S6K1 antibody
Western blot of Arabidopsis lysate showing specific immunolabeling of the ~53k S6K1 phosphorylated at Thr449. Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (P-peptide), but not by the corresponding nonphospho-peptide (NP-peptide).
anti-S6K1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for S6K1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
~53 kDa
NCBI Official Full Name
protein-serine kinase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 1; ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 6; ATPK1; ATPK6; ATS6K1; P70 RIBOSOMAL S6 KINASE; PK1; PK6; PROTEIN-SERINE KINASE; protein-serine kinase 1; ROTEIN-SERINE KINASE 6; S6K1
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NCBI Protein Information
protein-serine kinase 1
UniProt Protein Name
Serine/threonine-protein kinase AtPK1/AtPK6
UniProt Synonym Protein Names
Ribosomal-protein S6 kinase homolog 1
UniProt Gene Name
ATPK1  [Similar Products]
UniProt Synonym Gene Names
ATPK6; S6K1  [Similar Products]
UniProt Entry Name
KPK1_ARATH
NCBI Summary for S6K1
Encodes a protein-serine kinase that phosphorylates ribosomal protein in vitro. Activation of AtS6k is regulated by 1-naphthylacetic acid and kinetin, at least in part, via a lipid kinase-dependent pathway. Involved in translational up-regulation of ribosomal proteins. Phosphorylated by PDK1. Interacts with RAPTOR1, which in turn interacts with TOR. SPK6 activity is affected by osmotic stress, and plants overexpressing S6k1 are hypersensitive to osmotic stress. The gene is expressed in all tissues examined, with highest expression level detected in metabolically active tissues.
UniProt Comments for S6K1
Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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