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anti-CHAT antibody :: Goat ChAT (Choline Acetyltransferase) Polyclonal Antibody

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Catalog # MBS502147
Unit / Price
  0.1 mL  /  $360 +1 FREE 8GB USB
Testing Data
Product Name

ChAT (Choline Acetyltransferase), Polyclonal Antibody

Popular Item
Also Known As

Anti-Choline Acetyltransferase

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 10; NC_000010.11 (49609095..49665104). Location: 10q11.2
3D Structure
ModBase 3D Structure for P28329
Clonality
Polyclonal
Host
Goat
Species Reactivity
Avian, Chicken, Guinea Pig, Human, Mouse, non-Human primates
Specificity
Specific for the ~ 70k choline acetyltransferase protein
Purity/Purification
Affinity Purified (Prepared from goat serum by affinity purification using a column to which the protein immunogen was coupled.)
Form/Format
100 ul in PBS + 5 mg/ml BSA + 0.2% NaN3.. Adequate amount of material to conduct 10-mini Western Blots.
Antigen
Native choline acetyltransferase purified from human placenta
Immunogen Information
Native choline acetyltransferase purifed from human placenta
Immunogen Species
Human
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with chicken, guinea pig, human, mouse, non-human primate and rat tissues.
Biological Significance
Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).
Preparation and Storage
For long term storage -20 degree C is recommended. Stable for at least 1 year.
Other Notes
Small volumes of anti-CHAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CHAT antibody
Affinity purified goat polyclonal antibody
Applications Tested/Suitable for anti-CHAT antibody
Western Blot (WB)
Application Notes for anti-CHAT antibody
Quality Control: Western blots performed on each lot.
WB: 1:100

Testing Data of anti-CHAT antibody
anti-CHAT antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CHAT. It may not necessarily be applicable to this product.
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
70
NCBI Official Synonym Full Names
choline O-acetyltransferase
NCBI Official Symbol
NCBI Official Synonym Symbols
CMS1A; CMS1A2; CHOACTASE
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NCBI Protein Information
choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase
NCBI Summary for CHAT
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
UniProt Comments for CHAT
CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described.

Protein type: Acetyltransferase; Lipid Metabolism - glycerophospholipid; EC 2.3.1.6

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: cell soma; mitochondrion; axon; cytoplasm; cytosol; nucleus

Molecular Function: choline O-acetyltransferase activity

Biological Process: synaptic transmission; rhythmic excitation; muscle development; rhythmic behavior; phospholipid metabolic process; neurotransmitter secretion; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process; dendrite development; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; adult walking behavior; neurotransmitter biosynthetic process

Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea
Product References and Citations for anti-CHAT antibody
• Karson CN, Casanova MF, Kleinman JE, Griffin WS (1993) Choline acetyltransferase in schizophrenia. Am. J.Psychiatry 150:454-459.
• Holt DJ, Herman MM, Hyde TM, Kleinman JE, Cinton CM, German DC, Hersh LB, Greybiel AM, Saper CB (1999) Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia. Neuroscience 94(1):21-31.
• Baskin DS, Browning JL, Pirozzolo FJ, Korporaal S, Baskin JA, Appel SH (1999) Brain choline acetyltransferase and mental function in Alzheimer disease. Arch Neurol. 56:1121-1123.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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