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anti-NKIRAS2 antibody :: Rabbit NFkB, p65 Polyclonal Antibody

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Catalog # MBS631230
Unit / Price
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  0.1 mL  /  $420 +1 FREE 8GB USB
  0.25 mL  /  $470 +1 FREE 8GB USB
Product Name

NFkB, p65 (NKIRAS2), Polyclonal Antibody

Popular Item
Full Product Name

anti-Human NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA)

Product Synonym Names
Anti -NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 17; NC_000017.10 (40169594..40177659). Location: 17q21.2
OMIM
604497
3D Structure
ModBase 3D Structure for Q9NYR9
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Bovine, Canine, Drosophila, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Xenopus
Specificity
Recognizes human NF kappa B at ~65kD. Species Crossreactivity: mouse, rat, hamster, rabbit, bovine, porcine, canine, Drosophila and Xenopus.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Concentration
1mg/ml (lot specific)
Immunogen
Synthetic peptide from the C-terminus of human NF kappa B protein. Cellular Localization: Cytoplasmic.
Positive Control
Jurkat cells, Placenta
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Other Notes
Small volumes of anti-NKIRAS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NKIRAS2 antibody
NF kappa B is a heterodimer consisting of a 50kD DNA binding subunit and a 65kD transactivating subunit (RelA). The p50/p65 heterodime is present in the cytoplasm in inactive form bound to its inhibitor, IkB. Upon stimulation of cells with stimuli like LPS, Interleukin-1, IkB gets phosphorylated and degraded by proteosomes releasing the activated NFkB which goes to the nucleus inducing gene expression. NFkB was originally identified as transcriptional activator for kappa light cahain gene in B cells.
Applications Tested/Suitable for anti-NKIRAS2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-NKIRAS2 antibody
Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Dilution: Western Blot: 5-10ug/ml for 2 hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Immunohistochemistry of anti-NKIRAS2 antibody
Formalin-fixed, paraffin-embedded human placenta stained with MBS631230 using peroxidase-conjugate and AEC chromogen. Cytoplasmic staining of trophoblasts, fibroblasts and endothelial cells can be seen.
anti-NKIRAS2 antibody Immunohistochemistry (IHC) image
Western Blot of anti-NKIRAS2 antibody
Western Blot using MBS631230
anti-NKIRAS2 antibody Western Blot (WB) image
NCBI/Uniprot data below describe general gene information for NKIRAS2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,508 Da[Similar Products]
NCBI Official Full Name
NF-kappa-B inhibitor-interacting Ras-like protein 2 isoform c
NCBI Official Synonym Full Names
NFKB inhibitor interacting Ras-like 2
NCBI Official Symbol
NKIRAS2  [Similar Products]
NCBI Official Synonym Symbols
KBRAS2; kappaB-Ras2
  [Similar Products]
NCBI Protein Information
NF-kappa-B inhibitor-interacting Ras-like protein 2; kappa B-Ras protein 2; I-kappa-B-interacting Ras-like protein 2; NFKB inhibitor interacting Ras-like protein 2
UniProt Protein Name
NF-kappa-B inhibitor-interacting Ras-like protein 2
UniProt Synonym Protein Names
I-kappa-B-interacting Ras-like protein 2
UniProt Gene Name
NKIRAS2  [Similar Products]
UniProt Synonym Gene Names
KBRAS2; Kappa B-Ras protein 2; KappaB-Ras2  [Similar Products]
UniProt Entry Name
KBRS2_HUMAN
UniProt Comments for NKIRAS2
NKIRAS2: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF- kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, Ras; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: membrane; cytoplasm

Molecular Function: GTPase activity; GTP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; metabolic process; small GTPase mediated signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-NKIRAS2 antibody
Organs/Tissues associated with anti-NKIRAS2 antibody
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