anti-Gja1 antibody :: Rabbit CONNEXIN 43 Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

This item specifically recognises 43kDa Connexin43, when phosphorylated at Ser368. Connexin43 (CX43) allows for the exchange of solutions between neighbouring cells through the formation of gap-junctional channels and is expressed in the brain, kidneys and myocardium, as well as vascular endothelial cells. Studies suggest that a decrease in the permeability of gap-junctional channels occurs due to a conformational change in the structure of CX43, and that this is mediated by the phosphorylation of CX43 at residue Ser368, by Protein Kinase C (PKC). A possible link between CX43 and epilepsy has been investigated following the detection of high levels of CX43 in both low-grade tumours and peritumoural reactive astrocytes, see Aronica, E. et al. for details.

Purified
Purified IgG - liquid

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.

Small volumes of anti-Gja1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS223268 specifically recognises 43kDa Connexin43, when phosphorylated at Ser368. Connexin43 (CX43) allows for the exchange of solutions between neighbouring cells through the formation of gap-junctional channels and is expressed in the brain, kidneys and myocardium, as well as vascular endothelial cells. Studies suggest that a decrease in the permeability of gap-junctional channels occurs due to a conformational change in the structure of CX43, and that this is mediated by the phosphorylation of CX43 at residue Ser368, by Protein Kinase C (PKC). A possible link between CX43 and epilepsy has been investigated following the detection of high levels of CX43 in both low-grade tumours and peritumoural reactive astrocytes, see Aronica, E. et al. for details.

Western Blot (WB)*

Western Blot: This item detects a band of approximately 43kDa in rat hippocampus cell lysates.
Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

43,031 Da[Similar Products]

gap junction protein, alpha 1

gap junction alpha-1 protein; connexin 43; connexin-43; gap junction 43 kDa heart protein; gap junction membrane channel protein alpha 1

Gap junction alpha-1 protein

Cxn-43; Cx43  [Similar Products]

CXA1_RAT

gap junction component; plays a role in cell-cell communication [RGD, Feb 2006]

GJA1: an integral membrane protein of the connexin family, alpha-type (group II) subfamily. Hexamers of connexin-43 form connexons, which aggregate together to form gap junctions, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Channel, misc.; Membrane protein, integral

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; focal adhesion; contractile fiber; lysosome; integral to plasma membrane; early endosome; intermediate filament; fascia adherens; intercellular junction; cytosol; lipid raft; Golgi membrane; connexon complex; multivesicular body; mitochondrial outer membrane; membrane; cytoplasm; apical plasma membrane; late endosome; plasma membrane; gap junction; cell junction; endosome; lateral plasma membrane

Molecular Function: protein domain specific binding; protein binding; signal transducer activity; ion transmembrane transporter activity; beta-tubulin binding; SH3 domain binding; gap junction channel activity; receptor binding; PDZ domain binding

Biological Process: lens development in camera-type eye; response to peptide hormone stimulus; apoptosis; heart development; neuron migration; milk ejection; positive regulation of vasodilation; signal transduction; regulation of heart contraction; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; cell-cell signaling; positive regulation of glomerular filtration; negative regulation of cardiac muscle cell proliferation; response to glucose stimulus; positive regulation of striated muscle development; cell communication; heart looping; transmembrane transport; ATP transport; adult heart development; intercellular junction assembly and maintenance; chronic inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; gap junction assembly; epithelial cell maturation; in utero embryonic development; regulation of bone remodeling; positive regulation of insulin secretion; skeletal muscle regeneration; regulation of calcium ion transport; vascular transport; protein oligomerization; osteoblast differentiation; positive regulation of osteoblast differentiation; negative regulation of endothelial cell proliferation; positive regulation of vasoconstriction; positive regulation of protein catabolic process; embryonic heart tube development; blood vessel morphogenesis; embryonic digit morphogenesis; regulation of bone mineralization; neurite morphogenesis; response to pH

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