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anti-CALM1 antibody :: Rabbit CALM1 Polyclonal Antibody

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Catalog # MBS9214524
Unit / Price
  0.08 mL  /  $150 +1 FREE 8GB USB
  0.4 mL  /  $340 +1 FREE 8GB USB
Product Name

CALM1, Polyclonal Antibody

Popular Item
Also Known As

CALM1 Antibody (C-term)

Product Synonym Names
Calmodulin; CaM; CALM1; CALM; CAM; CAM1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
107-132
Immunogen Sequence Length
149
OMIM
114180
3D Structure
ModBase 3D Structure for P62158
Clonality
Polyclonal
Isotype
Rabbit Ig
Clone Number
RB21103
Host
Rabbit
Species Reactivity
Human (Predicted Reactivity: Bovine, C Elegans, Chicken, Zebrafish, Drosophila, Mouse, Rabbit, Rat, Xenopus)
Specificity
This CALM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-132 amino acids from the C-terminal region of human CALM1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Cellular location
Cytoplasm, cytoskeleton, spindle. Cytoplasm,cytoskeleton, spindle pole. Note = Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules
Function
Function Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-CALM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CALM1 antibody
CALM1 is a member of the EF-hand calcium-binding protein family.Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Applications Tested/Suitable for anti-CALM1 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-CALM1 antibody
WB~~1:1000
FC~~1:10~50
IHC-P~~1:50~100
IF~~1:10~50

Western Blot (WB) of anti-CALM1 antibody
Western blot analysis of CALM1 Antibody (C-term) in Hela cell line lysates (35ug/lane). CALM1 (arrow) was detected using the purified Pab.(2ug/ml)
anti-CALM1 antibody Western Blot (WB) (WB) image
Flow Cytometry (FC/FACS) of anti-CALM1 antibody
CALM1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-CALM1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
Immunohistochemistry (IHC) of anti-CALM1 antibody
CALM1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-CALM1 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-CALM1 antibody
Confocal immunofluorescent analysis of CALM1 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
anti-CALM1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for CALM1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Human (Predicted Reactivity: Bovine, C Elegans, Chicken, Zebrafish, Drosophila, Mouse, Rabbit, Rat, Xenopus)NP_005175.2; NP_008819.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16838
NCBI Official Full Name
calmodulin isoform 2
NCBI Official Synonym Full Names
calmodulin 2 (phosphorylase kinase, delta)
NCBI Official Symbol
CALM2  [Similar Products]
NCBI Official Synonym Symbols
caM; PHKD; CAMII; LQT15; PHKD2
  [Similar Products]
NCBI Protein Information
calmodulin
UniProt Protein Name
Calmodulin
Protein Family
UniProt Gene Name
CALM1  [Similar Products]
UniProt Synonym Gene Names
CALM; CAM; CAM1; CaM  [Similar Products]
UniProt Entry Name
CALM_HUMAN
NCBI Summary for CALM1
This gene is a member of the calmodulin gene family. There are three distinct calmodulin genes dispersed throughout the genome that encode the identical protein, but differ at the nucleotide level. Calmodulin is a calcium binding protein that plays a role in signaling pathways, cell cycle progression and proliferation. Several infants with severe forms of long-QT syndrome (LQTS) who displayed life-threatening ventricular arrhythmias together with delayed neurodevelopment and epilepsy were found to have mutations in either this gene or another member of the calmodulin gene family (PMID:23388215). Mutations in this gene have also been identified in patients with less severe forms of LQTS (PMID:24917665), while mutations in another calmodulin gene family member have been associated with catecholaminergic polymorphic ventricular tachycardia (CPVT)(PMID:23040497), a rare disorder thought to be the cause of a significant fraction of sudden cardiac deaths in young individuals. Pseudogenes of this gene are found on chromosomes 10, 13, and 17. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]
UniProt Comments for CALM1
Calmodulin: a calcium-binding small regulatory protein that mediates the control of a large number of enzymes by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Has four functional calcium-binding domains. Phosphorylation and ubiquitination result in a decreased activity.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 14q32.11

Cellular Component: nucleoplasm; spindle pole; centrosome; sarcomere; growth cone; spindle microtubule; cytoplasm; plasma membrane; extracellular region; cytosol; nucleus; vesicle

Molecular Function: protein domain specific binding; nitric-oxide synthase regulator activity; titin binding; calcium ion binding; calcium-dependent protein binding; protein kinase binding; protein serine/threonine kinase activator activity; protein binding; N-terminal myristoylation domain binding; phospholipase binding; thioesterase binding; type 3 metabotropic glutamate receptor binding; phosphoinositide 3-kinase binding; nitric-oxide synthase binding; adenylate cyclase binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; phototransduction, visible light; inositol phosphate metabolic process; nerve growth factor receptor signaling pathway; pathogenesis; signal transduction; nitric oxide metabolic process; positive regulation of protein amino acid dephosphorylation; synaptic transmission; platelet degranulation; substantia nigra development; muscle contraction; positive regulation of cyclic nucleotide metabolic process; response to corticosterone stimulus; epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; platelet activation; fibroblast growth factor receptor signaling pathway; glycogen catabolic process; regulation of heart rate; positive regulation of nitric-oxide synthase activity; organelle organization and biogenesis; response to amphetamine; adenylate cyclase activation; glucose metabolic process; regulation of cytokinesis; positive regulation of protein amino acid autophosphorylation; rhodopsin mediated signaling; G-protein coupled receptor protein signaling pathway; regulation of rhodopsin mediated signaling; phospholipase C activation; carbohydrate metabolic process; innate immune response; positive regulation of cyclic-nucleotide phosphodiesterase activity; detection of calcium ion; regulation of nitric-oxide synthase activity; vascular endothelial growth factor receptor signaling pathway; blood coagulation; response to calcium ion

Disease: Long Qt Syndrome 14; Ventricular Tachycardia, Catecholaminergic Polymorphic, 4
Product References and Citations for anti-CALM1 antibody
Zhao,D., et.al., Zhonghua Yi Xue Za Zhi 88 (35), 2452-2456 (2008)
Martins-de-Souza,D., et.al., J. Neural Transm. 116 (3), 275-289 (2009)

Precautions
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Disclaimer
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