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anti-SMAD2 antibody :: Rabbit SMAD2 Polyclonal Antibody

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Catalog # MBS8205104
Unit / Price
  0.03 mL  /  $165 +1 FREE 8GB USB
  0.1 mL  /  $245 +1 FREE 8GB USB
  0.2 mL  /  $350 +1 FREE 8GB USB
Product Name

SMAD2, Polyclonal Antibody

Popular Item
Full Product Name

Anti-SMAD2 (pS465) Antibody

Product Synonym Names
MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2
Antibody/Peptide Pairs
SMAD2 peptide (MBS8237172) is used for blocking the activity of SMAD2 antibody (MBS8205104)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
601366
3D Structure
ModBase 3D Structure for Q15796
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rabbit, Bovine, Chicken, Monkey, Porcine, Rabbit, Sheep, Zebrafish
Specificity
Recognizes endogenous levels of SMAD2 (pS465) protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SMAD2 (pS465). The exact sequence is proprietary.
Source
Rabbit
Preparation and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-SMAD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SMAD2 antibody
Rabbit polyclonal antibody to SMAD2 (pS465)
Applications Tested/Suitable for anti-SMAD2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SMAD2 antibody
WB (1/500 - 1/1000)
IHC (1/100 - 1/200)

Western Blot (WB) of anti-SMAD2 antibody
Western blot analysis of SMAD2 (pS465) expression in HepG2 (A) whole cell lysates.
anti-SMAD2 antibody Western Blot (WB) (WB) image
Immunohistochemical of anti-SMAD2 antibody
Immunohistochemical analysis of SMAD2 (pS465) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-SMAD2 antibody Immunohistochemical image
NCBI/Uniprot data below describe general gene information for SMAD2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
mothers against decapentaplegic homolog 2 isoform 1
NCBI Official Synonym Full Names
SMAD family member 2
NCBI Official Symbol
SMAD2  [Similar Products]
NCBI Official Synonym Symbols
JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
  [Similar Products]
NCBI Protein Information
mothers against decapentaplegic homolog 2
UniProt Protein Name
Mothers against decapentaplegic homolog 2
UniProt Synonym Protein Names
JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2
UniProt Gene Name
SMAD2  [Similar Products]
UniProt Synonym Gene Names
MADH2; MADR2; MAD homolog 2; Mothers against DPP homolog 2; hMAD-2; SMAD 2; Smad2; hSMAD2  [Similar Products]
UniProt Entry Name
SMAD2_HUMAN
NCBI Summary for SMAD2
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
UniProt Comments for SMAD2
SMAD2: ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; cytosol; nucleus

Molecular Function: transcription activator binding; metal ion binding; transcription factor binding; phosphatase binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; chromatin binding; SMAD binding; transforming growth factor beta receptor binding; transcription factor activity

Biological Process: developmental growth; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; gastrulation; negative regulation of transcription from RNA polymerase II promoter; palate development; primary microRNA processing; post-embryonic development; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; ureteric bud development; transforming growth factor beta receptor signaling pathway; embryonic foregut morphogenesis; pancreas development; response to glucose stimulus; positive regulation of BMP signaling pathway; pericardium development; transcription initiation from RNA polymerase II promoter; cell fate commitment; regulation of binding; transcription, DNA-dependent; in utero embryonic development; common-partner SMAD protein phosphorylation; embryonic cranial skeleton morphogenesis; SMAD protein complex assembly; organ growth; zygotic determination of dorsal/ventral axis; mesoderm formation; insulin secretion; endoderm formation; positive regulation of transcription from RNA polymerase II promoter; gene expression; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of transcription, DNA-dependent; lung development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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