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anti-YWHAE antibody :: Rabbit 14-3-3 epsilon Polyclonal Antibody

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Catalog # MBS820834
Unit / Price
  0.03 mL  /  $155 +1 FREE 8GB USB
  0.1 mL  /  $220 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Immunofluorescence/Immunocytochemistry (IF/ICC)
Product Name

14-3-3 epsilon (YWHAE), Polyclonal Antibody

Also Known As

Anti-14-3-3 epsilon Antibody

Product Synonym Names
14-3-3 protein epsilon; 14-3-3E
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
255
OMIM
605066
3D Structure
ModBase 3D Structure for P62258
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Chicken, Monkey, Sheep, Zebrafish
Specificity
Recognizes endogenous levels of 14-3-3 epsilon protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human 14-3-3 epsilon. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-YWHAE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-YWHAE antibody
Rabbit polyclonal antibody to 14-3-3 epsilon
Applications Tested/Suitable for anti-YWHAE antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)
Application Notes for anti-YWHAE antibody
WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-YWHAE antibody
anti-YWHAE antibody Western Blot (WB) (WB) image
Immunofluorescence/Immunocytochemistry (IF/ICC) of anti-YWHAE antibody
Immunofluorescent analysis of 14-3-3 epsilon staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-YWHAE antibody Immunofluorescence/Immunocytochemistry (IF/ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for YWHAE. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
26,504 Da
NCBI Official Full Name
14-3-3 protein epsilon
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
NCBI Official Symbol
YWHAE  [Similar Products]
NCBI Official Synonym Symbols
MDS; HEL2; MDCR; KCIP-1; 14-3-3E
  [Similar Products]
NCBI Protein Information
14-3-3 protein epsilon; 14-3-3 epsilon; epididymis luminal protein 2; protein kinase C inhibitor protein-1; mitochondrial import stimulation factor L subunit; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
UniProt Protein Name
14-3-3 protein epsilon
Protein Family
UniProt Gene Name
YWHAE  [Similar Products]
UniProt Synonym Gene Names
14-3-3E  [Similar Products]
UniProt Entry Name
1433E_HUMAN
NCBI Summary for YWHAE
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Comments for YWHAE
14-3-3 epsilon: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: kinesin complex; focal adhesion; cytoplasmic vesicle membrane; mitochondrion; membrane; axon; melanosome; cytosol

Molecular Function: protein domain specific binding; protein binding; potassium channel regulator activity; enzyme binding; protein heterodimerization activity; histone deacetylase binding; phosphoprotein binding; phosphoserine binding

Biological Process: substantia nigra development; nerve growth factor receptor signaling pathway; viral reproduction; apoptosis; organelle organization and biogenesis; hippocampus development; neuron migration; cerebral cortex development; mitotic cell cycle; regulation of caspase activity; G2/M transition of mitotic cell cycle; regulation of the rate of heart contraction by hormone; protein targeting

Disease: Miller-dieker Lissencephaly Syndrome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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