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anti-YWHAG antibody :: Rabbit 14-3-3 gamma Polyclonal Antibody

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Catalog # MBS820681
Unit / Price
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  0.03 mL  /  $155 +1 FREE 8GB USB
  0.1 mL  /  $220 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Immunofluorescence/Immunocytochemistry (IF/ICC)
Product Name

14-3-3 gamma (YWHAG), Polyclonal Antibody

Full Product Name

Anti-14-3-3 gamma Antibody

Product Synonym Names
14-3-3 protein gamma; Protein kinase C inhibitor protein 1; KCIP-1
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
605356
3D Structure
ModBase 3D Structure for P61981
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Chicken, Zebrafish
Specificity
Recognizes endogenous levels of 14-3-3 gamma protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 14-3-3 gamma. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-YWHAG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-YWHAG antibody
Rabbit polyclonal antibody to 14-3-3 gamma
Applications Tested/Suitable for anti-YWHAG antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)
Application Notes for anti-YWHAG antibody
WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-YWHAG antibody
anti-YWHAG antibody Western Blot (WB) (WB) image
Immunofluorescence/Immunocytochemistry (IF/ICC) of anti-YWHAG antibody
Immunofluorescent analysis of 14-3-3 gamma staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-YWHAG antibody Immunofluorescence/Immunocytochemistry (IF/ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for YWHAG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,303 Da
NCBI Official Full Name
14-3-3 protein gamma
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma
NCBI Official Symbol
YWHAG  [Similar Products]
NCBI Official Synonym Symbols
PPP1R170; 14-3-3GAMMA
  [Similar Products]
NCBI Protein Information
14-3-3 protein gamma; KCIP-1; 14-3-3 gamma; protein kinase C inhibitor protein 1; protein phosphatase 1, regulatory subunit 170; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
UniProt Protein Name
14-3-3 protein gamma
UniProt Synonym Protein Names
Protein kinase C inhibitor protein 1; KCIP-1
Protein Family
UniProt Gene Name
YWHAG  [Similar Products]
UniProt Synonym Gene Names
KCIP-1  [Similar Products]
UniProt Entry Name
1433G_HUMAN
NCBI Summary for YWHAG
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]
UniProt Comments for YWHAG
14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: focal adhesion; cytoplasmic vesicle membrane; membrane; cytosol

Molecular Function: protein kinase C inhibitor activity; protein domain specific binding; insulin-like growth factor receptor binding; protein binding; protein kinase C binding; receptor tyrosine kinase binding

Biological Process: cellular response to insulin stimulus; regulation of neuron differentiation; apoptosis; organelle organization and biogenesis; regulation of signal transduction; regulation of synaptic plasticity; negative regulation of protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein targeting
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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