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anti-14-3-3 ZETA/DELTA antibody :: Rabbit 14-3-3 ZETA/DELTA Polyclonal Antibody

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Catalog # MBS222336
Unit / Price
  0.1 mL  /  $490 +1 FREE 8GB USB
Testing Data #1
Product Name

14-3-3 ZETA/DELTA, Polyclonal Antibody

Also Known As

RABBIT ANTI 14-3-3 ZETA/DELTA

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
245
3D Structure
ModBase 3D Structure for P29361
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Bovine, Chicken, Human, Mammals (Expected from Sequence), Mouse, Rabbit, Rat
Specificity
This item recognizes the acetylated N-terminal of 14-3-3 zeta and the phosphorylated zeta isoform (also known as 14-3-3 delta) in all mammals. These are members of the 14-3-3 family which consists of 30kDa proteins that are involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 zeta is a susceptibility gene for paranoid schizophrenia and is overexpressed in the temporal cortex of cognitively impared Alzheimers patients. The protein binds yeast Sps1/Ste20-related kinase 1 (YSK1) and localizes to the golgi, possibly linking YSK1 signaling, protein transport, cell adhesion and migration. It co-localizes with epidermal growth factor receptor to the plasma membrane following epidermal growth factor signaling. The zeta isoform is involved in MAPKAPK2-mediated phosphorylation which may have a role in p38 MAPK-dependent inflammation. When bound to ADAM 22, 14-3-3 zeta is involved in cell adhesion. 14-3-3 zeta also interacts with cofilin, LIM-domain-containing protein kinase 1, protein kinase B/Akt and GPI alpha. 14-3-3 zeta can be phosphorylated at serine 184 to produce 14-3-3 delta.This item may not react with recombinant proteins that are not N-acetylated.
Form/Format
Serum
Serum - liquid
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 zeta/delta. Peptide sequence: Ac.MDKNELVQKAC
Histology Positive Control Tissue
Antiserum Preparation
Antisera to anti 14-3-3 zeta/delta were raised by repeated immunisations of rabbits with highly purified antigen.
Target Species
Sheep
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-14-3-3 ZETA/DELTA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-14-3-3 ZETA/DELTA antibody
Rabbit anti 14-3-3ζ antibody recognizes the acetylated N-terminal of 14-3-3 zeta and the phosphorylated zeta isoform (also known as 14-3-3 delta) in all mammals. These are members of the 14-3-3 family which consists of 30 kDa proteins involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3ζ is a susceptibility gene for paranoid schizophrenia and is overexpressed in the temporal cortex of cognitively impared Alzheimers patients. The protein binds yeast Sps1/Ste20-related kinase 1 (YSK1) and localizes to the golgi, possibly linking YSK1 signaling, protein transport, cell adhesion and migration. It co-localizes with epidermal growth factor receptor to the plasma membrane following epidermal growth factor signaling. The zeta isoform is involved in MAPKAPK2-mediated phosphorylation which may have a role in p38 MAPK-dependent inflammation. When bound to ADAM 22, 14-3-3 zeta is involved in cell adhesion. 14-3-3ζ also interacts with cofilin, LIM-domain-containing protein kinase 1, protein kinase B/Akt and GPI alpha. 14-3-3ζ can be phosphorylated at serine 184 to produce 14-3-3 delta. Rabbit anti 14-3-3ζ antibody may not react with recombinant proteins that are not N-acetylated.
Applications Tested/Suitable for anti-14-3-3 ZETA/DELTA antibody
ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-14-3-3 ZETA/DELTA antibody
Western Blot: This item detects a band of approximately 30kDa in HEK293 cell lysates.
Immunohistology - Paraffin: Maximum Dilution: 1/400; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Western Blotting: Maximum Dilution: 1/3000

Western blots of 14-3-3 isoforms in HEK293 cell extracts using our antibodies.
Lane 1 = anti-gamma 14-3-3 (MBS222332)
Lane 2 = anti-zeta 14-3-3 (MBS222336)
Lane 3 = anti-C-epsilon-14-3-3 (MBS222330)
Lane 4 = anti-N-epsilon-14-3-3 (MBS222333)
Lane 5 = anti-eta 14-3-3. (MBS222331)
The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3
anti-14-3-3 ZETA/DELTA antibody Testing Data #1 image
NCBI/Uniprot data below describe general gene information for 14-3-3 ZETA/DELTA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
27,856 Da
NCBI Official Full Name
14-3-3 protein zeta/delta
UniProt Protein Name
14-3-3 protein zeta/delta
UniProt Synonym Protein Names
Protein kinase C inhibitor protein 1; KCIP-1
UniProt Gene Name
YWHAZ  [Similar Products]
UniProt Synonym Gene Names
KCIP-1  [Similar Products]
UniProt Entry Name
1433Z_SHEEP
UniProt Comments for 14-3-3 ZETA/DELTA
14-3-3 zeta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Phosphorylation apparently disrupts homodimerization.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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