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anti-CAMK2A antibody :: Rabbit CaMK2 alpha/delta Polyclonal Antibody

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Catalog # MBS8218549
Unit / Price
  0.03 mL  /  $165 +1 FREE 8GB USB
  0.1 mL  /  $245 +1 FREE 8GB USB
  0.2 mL  /  $350 +1 FREE 8GB USB
Product Name

CaMK2 alpha/delta (CAMK2A), Polyclonal Antibody

Popular Item
Also Known As

Anti-CaMK2 alpha/delta (pT286) Antibody

Product Synonym Names
CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2D; CAMKD; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II subunit delta; CaMK-II subunit delta
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
478
OMIM
114078
3D Structure
ModBase 3D Structure for Q9UQM7
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Chicken, Monkey, Porcine, Rabbit
Specificity
Recognizes endogenous levels of CaMK2 alpha/delta (pT286) protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CaMK2 alpha/delta. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-CAMK2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CAMK2A antibody
Rabbit polyclonal antibody to CaMK2 alpha/delta (pT286)
Applications Tested/Suitable for anti-CAMK2A antibody
Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-CAMK2A antibody
WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF (1/100 - 1/500), ICC (1/100 - 1/500)

Western Blot (WB) of anti-CAMK2A antibody
Western blot analysis of CaMK2 alpha/delta (pT286) expression in HeLa (A) whole cell lysates.
anti-CAMK2A antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CAMK2A antibody
Immunohistochemical analysis of CaMK2 alpha/delta (pT286) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. w
anti-CAMK2A antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-CAMK2A antibody
Immunofluorescent analysis of CaMK2 alpha/delta (pT286) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-CAMK2A antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for CAMK2A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55,320 Da
NCBI Official Full Name
calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 2
NCBI Official Synonym Full Names
calcium/calmodulin-dependent protein kinase II alpha
NCBI Official Symbol
CAMK2A  [Similar Products]
NCBI Official Synonym Symbols
CAMKA
  [Similar Products]
NCBI Protein Information
calcium/calmodulin-dependent protein kinase type II subunit alpha
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type II subunit alpha
UniProt Gene Name
CAMK2A  [Similar Products]
UniProt Synonym Gene Names
CAMKA; KIAA0968; CaM kinase II subunit alpha; CaMK-II subunit alpha  [Similar Products]
UniProt Entry Name
KCC2A_HUMAN
NCBI Summary for CAMK2A
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]
UniProt Comments for CAMK2A
CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.17; Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: nucleoplasm; presynaptic membrane; mitochondrion; plasma membrane; cytosol; nucleus; cell junction

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; protein binding; glutamate receptor binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; kinase activity; ATP binding

Biological Process: synaptic transmission; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; calcium ion transport; regulation of neurotransmitter secretion; positive regulation of calcium ion transport; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle; regulation of neuronal synaptic plasticity; activation of NF-kappaB transcription factor
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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