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anti-COL4A1 antibody :: Rabbit Collagen 4 alpha 1 Polyclonal Antibody

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Catalog # MBS821895
Unit / Price
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  0.03 mL  /  $155 +1 FREE 8GB USB
  0.1 mL  /  $220 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

Collagen 4 alpha 1 (COL4A1), Polyclonal Antibody

Full Product Name

Anti-Collagen 4 alpha 1 Antibody

Product Synonym Names
Collagen alpha-1(IV) chain
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
phenotype 611773
3D Structure
ModBase 3D Structure for P02462
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Bovine, Chicken, Pig
Specificity
Recognizes endogenous levels of Collagen 4 alpha 1 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 4 alpha 1. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-COL4A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-COL4A1 antibody
Rabbit polyclonal antibody to Collagen 4 alpha 1
Applications Tested/Suitable for anti-COL4A1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-COL4A1 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200)

Western Blot (WB) of anti-COL4A1 antibody
anti-COL4A1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-COL4A1 antibody
Immunohistochemical analysis of Collagen 4 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-COL4A1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for COL4A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
127,981 Da
NCBI Official Full Name
collagen alpha-1(IV) chain preproprotein
NCBI Official Synonym Full Names
collagen, type IV, alpha 1
NCBI Official Symbol
COL4A1  [Similar Products]
NCBI Official Synonym Symbols
ICH; HANAC; POREN1; arresten
  [Similar Products]
NCBI Protein Information
collagen alpha-1(IV) chain; collagen alpha-1(IV) chain; COL4A1 NC1 domain; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain
UniProt Protein Name
Collagen alpha-1(IV) chain
Protein Family
UniProt Gene Name
COL4A1  [Similar Products]
UniProt Entry Name
CO4A1_HUMAN
NCBI Summary for COL4A1
This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jul 2008]
UniProt Comments for COL4A1
COL4A1: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH). Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant. Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC). The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries. Defects in COL4A1 are a cause of familial porencephaly (POREN1). Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles. Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Extracellular matrix

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: extracellular matrix; endoplasmic reticulum lumen; collagen type IV; extracellular region; basement membrane

Molecular Function: protein binding; platelet-derived growth factor binding; extracellular matrix structural constituent; extracellular matrix constituent conferring elasticity

Biological Process: patterning of blood vessels; extracellular matrix disassembly; collagen catabolic process; receptor-mediated endocytosis; axon guidance; extracellular matrix organization and biogenesis; epithelial cell differentiation; blood vessel morphogenesis; brain development; neuromuscular junction development

Disease: Porencephaly 1; Brain Small Vessel Disease With Or Without Ocular Anomalies; Angiopathy, Hereditary, With Nephropathy, Aneurysms, And Muscle Cramps; Retinal Arteries, Tortuosity Of; Hemorrhage, Intracerebral, Susceptibility To
Precautions
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Disclaimer
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