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anti-LCK antibody :: Rabbit LCK (pY393) Polyclonal Antibody

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Catalog # MBS822038
Unit / Price
  0.03 mL  /  $165 +1 FREE 8GB USB
  0.1 mL  /  $245 +1 FREE 8GB USB
  0.2 mL  /  $350 +1 FREE 8GB USB
Immunofluorescence/Immunocytochemistry (IF/ICC)
Product Name

LCK (pY393), Polyclonal Antibody

Popular Item
Full Product Name

Anti-LCK (pY393) Antibody

Product Synonym Names
Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
Product Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 615758
3D Structure
ModBase 3D Structure for P06239
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep
Specificity
Recognizes endogenous levels of LCK (pY393) protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LCK. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-LCK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-LCK antibody
Rabbit polyclonal antibody to LCK (pY393)
Applications Tested/Suitable for anti-LCK antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)
Application Notes for anti-LCK antibody
WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-LCK antibody
anti-LCK antibody Western Blot (WB) (WB) image
Immunofluorescence/Immunocytochemistry (IF/ICC) of anti-LCK antibody
Immunofluorescent analysis of LCK (pY393) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-LCK antibody Immunofluorescence/Immunocytochemistry (IF/ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for LCK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
61,190 Da
NCBI Official Full Name
tyrosine-protein kinase Lck
NCBI Official Synonym Full Names
LCK proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
LSK; YT16; IMD22; p56lck; pp58lck
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NCBI Protein Information
tyrosine-protein kinase Lck; leukocyte C-terminal Src kinase; p56(LSTRA) protein-tyrosine kinase; t cell-specific protein-tyrosine kinase; proto-oncogene tyrosine-protein kinase LCK; lymphocyte-specific protein tyrosine kinase; lymphocyte cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck
UniProt Protein Name
Tyrosine-protein kinase Lck
UniProt Synonym Protein Names
Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
LCK_HUMAN
NCBI Summary for LCK
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq, Jul 2008]
UniProt Comments for LCK
Lck: a tyrosine kinase of the Src family that is crucial to antigen-receptor signaling in lymphocytes. plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Binds to the cytoplasmic domain of cell surface receptors, such as CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to other protein kinases including CDC2, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of Sam68 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Overexpression in mice leads to thymic tumors. Aberrant expression is seen in T cell leukemias and colon cancer. The leukemic translocation t(1;7)(p34;q34) has breakpoints at the T cell receptor gene and close to the Lck promoters, can cause increased Lck expression, and in one case, point mutations. A mutated Lck has also been seen in a cell line. One patient with aberrant Lck splicing suffered from SCID-like T cell deficiency. Inhibitor: BMS-279700. Three alternatively spliced isoforms of the human proteinhave been described.

Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Oncoprotein; TK group; Src family

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: pericentriolar material; extrinsic to internal side of plasma membrane; endocytic vesicle; plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft

Molecular Function: protein C-terminus binding; identical protein binding; CD8 receptor binding; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine phosphatase activity; protein kinase binding; antigen binding; protein phosphatase binding; ATPase binding; protein binding; protein-tyrosine kinase activity; protein complex binding; phosphoinositide 3-kinase binding; SH2 domain binding; glycoprotein binding; CD4 receptor binding; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; viral reproduction; protein amino acid phosphorylation; T cell receptor signaling pathway; B cell receptor signaling pathway; positive regulation of T cell receptor signaling pathway; hemopoiesis; positive regulation of gamma-delta T cell differentiation; T cell differentiation; aging; epidermal growth factor receptor signaling pathway; response to drug; caspase activation; regulation of lymphocyte activation; platelet activation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; cellular zinc ion homeostasis; regulation of defense response to virus by virus; regulation of cell proliferation; positive regulation of tyrosine phosphorylation of Stat5 protein; response to hydrogen peroxide; dephosphorylation; response to mechanical stimulus; response to zinc ion; release of sequestered calcium ion into cytosol; T cell costimulation; innate immune response; positive regulation of T cell activation; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Immunodeficiency 22
Precautions
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Disclaimer
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