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anti-TUBG1 antibody :: Rabbit Tubulin-gamma Polyclonal Antibody

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Catalog # MBS301666
Unit / Price DISCONTINUED
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Immunohistochemistry (IHC)
Product Name

Tubulin-gamma (TUBG1), Polyclonal Antibody

Also Known As

Rabbit Anti-Human Tubulin-gamma Polyclonal Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
451
OMIM
191135
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Predicted Reactivity: Bovine, Dog, Frog, Mouse, Rat
Form/Format
0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
Immunogen
Synthetic peptide derived from C-terminus of human tubulin gamma protein.
Control Tissue
Colon Carcinoma
Cellular Localization
Cytoplasm
Entrez Gene Code
7283
Pathway
Cytoskeletal Proteins
Preparation and Storage
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
Other Notes
Small volumes of anti-TUBG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TUBG1 antibody
Tubulin gamma, an essential structural protein for cell growth, organizes microtubule arrays in the nucleus and cytoplasm. Tubulin gamma-depleted cells fail to form functional spindles and arrest during nuclear division. Tubulin gamma associates with spindle body components Spc97 and Spc98 to form the tubulin gamma complex. The phosphorylation of tubulin gamma at Tyr445 plays a regulatory role in microtubule formation. The incidence rate for this phosphorylation event peaks during G1.
Applications Tested/Suitable for anti-TUBG1 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-TUBG1 antibody
Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:100
IHC Positive Control: Colon Carcinoma

Immunohistochemistry (IHC) of anti-TUBG1 antibody
Human colon carcinoma stained with anti-tubulin gamma antibody
anti-TUBG1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for TUBG1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
NCBI Official Full Name
tubulin gamma-1 chain
NCBI Official Synonym Full Names
tubulin, gamma 1
NCBI Official Symbol
TUBG1  [Similar Products]
NCBI Official Synonym Symbols
TUBG; GCP-1; CDCBM4; TUBGCP1
  [Similar Products]
NCBI Protein Information
tubulin gamma-1 chain; gamma-tubulin complex component 1; tubulin, gamma polypeptide
UniProt Protein Name
Tubulin gamma-1 chain
UniProt Synonym Protein Names
Gamma-1-tubulin; Gamma-tubulin complex component 1; GCP-1
UniProt Gene Name
TUBG1  [Similar Products]
UniProt Synonym Gene Names
TUBG; GCP-1  [Similar Products]
UniProt Entry Name
TBG1_HUMAN
NCBI Summary for TUBG1
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]
UniProt Comments for TUBG1
TUBG1: Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO/C1orf88. Belongs to the tubulin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: centriole; centrosome; pericentriolar material; recycling endosome; condensed nuclear chromosome; cytoplasmic microtubule; apical part of cell; nonmotile primary cilium; leading edge; cytoplasm; polar microtubule; cytosol; gamma-tubulin complex

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: metabolic process; organelle organization and biogenesis; microtubule cytoskeleton organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; microtubule nucleation; meiotic spindle organization and biogenesis; cytoplasmic microtubule organization and biogenesis

Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 4
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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